High diversity of blaNDM-1 -encoding plasmids in Klebsiella pneumoniae isolated from neonates in a Vietnamese hospital
Journal article, 2022
Objectives: The carbapenemase-encoding gene blaNDM-1 has been reported in Vietnam during the last 10 years, and blaNDM-producing Enterobacteriaceae are now silently and rapidly spreading. A key factor behind dissemination of blaNDM-1 is plasmids, mobile genetic elements that commonly carry antibiotic resistance genes and spread via conjugation. The diversity of blaNDM-1-encoding plasmids from neonates at a large Vietnamese hospital was characterized in this study. Methods: 18 fecal Klebsiella pneumoniae and Klebsiella quasipneumoniae isolates collected from 16 neonates at a large pediatric hospital in Vietnam were studied using optical DNA mapping (ODM) and next-generation sequencing (NGS). Plasmids carrying the blaNDM-1 gene were identified by combining ODM with Cas9 restriction. The plasmids in the isolates were compared to investigate whether the same plasmid was present in different patients. Results: Although the same plasmid was found in some isolates, ODM confirmed that there were at least 10 different plasmids encoding blaNDM-1 among the 18 isolates, thus indicating wide plasmid diversity. The ODM results concur with the NGS data. Interestingly, some isolates had two distinct plasmids encoding blaNDM-1 that could be readily identified with ODM. The coexistence of different plasmids carrying the same blaNDM-1 gene in a single isolate has rarely been reported, probably because of limitations in plasmid characterization techniques. Conclusions: The plasmids encoding the blaNDM-1 gene in this study cohort were diverse and may represent a similar picture in Vietnamese society. The study highlights important aspects of the usefulness of ODM for plasmid analysis.
Optical DNA mapping
Plasmids
Carbapenem
bla NDM-1
Author
Sriram Kesarimangalam
Chalmers, Biology and Biological Engineering, Chemical Biology
Elina Ekedahl
Chalmers, Biology and Biological Engineering, Chemical Biology
Ngoc Thi Bich Hoang
Vietnam National Children's Hospital
Tsegaye Sewunet
Karolinska Institutet
Björn Berglund
Linköping University
Ludwig Lundberg
Swedish University of Agricultural Sciences (SLU)
Linköping University
Shoeib Nematzadeh
Karolinska Institutet
Maud Nilsson
Linköping University
Lennart E. Nilsson
Linköping University
Ngai Kien Le
Vietnam National Children's Hospital
Tran Minh Dien
Vietnam National Children's Hospital
Håkan Hanberger
Linköping University
Linus Olson
Karolinska Institutet
Mattias Larsson
Karolinska Institutet
Christian G. Giske
Karolinska University Hospital
Karolinska Institutet
Fredrik Westerlund
Chalmers, Biology and Biological Engineering, Chemical Biology
International Journal of Antimicrobial Agents
0924-8579 (ISSN) 1872-7913 (eISSN)
Vol. 59 2 106496Areas of Advance
Nanoscience and Nanotechnology
Health Engineering
Subject Categories (SSIF 2011)
Microbiology
Medical Genetics
Genetics
Infrastructure
Myfab (incl. Nanofabrication Laboratory)
DOI
10.1016/j.ijantimicag.2021.106496
PubMed
34921976