High diversity of blaNDM-1-encoding plasmids in Klebsiella pneumoniae isolated from neonates in a Vietnamese hospital
Artikel i vetenskaplig tidskrift, 2022

Objectives: The carbapenemase-encoding gene blaNDM-1 has been reported in Vietnam during the last 10 years, and blaNDM-producing Enterobacteriaceae are now silently and rapidly spreading. A key factor behind dissemination of blaNDM-1 is plasmids, mobile genetic elements that commonly carry antibiotic resistance genes and spread via conjugation. The diversity of blaNDM-1-encoding plasmids from neonates at a large Vietnamese hospital was characterized in this study. Methods: 18 fecal Klebsiella pneumoniae and Klebsiella quasipneumoniae isolates collected from 16 neonates at a large pediatric hospital in Vietnam were studied using optical DNA mapping (ODM) and next-generation sequencing (NGS). Plasmids carrying the blaNDM-1 gene were identified by combining ODM with Cas9 restriction. The plasmids in the isolates were compared to investigate whether the same plasmid was present in different patients. Results: Although the same plasmid was found in some isolates, ODM confirmed that there were at least 10 different plasmids encoding blaNDM-1 among the 18 isolates, thus indicating wide plasmid diversity. The ODM results concur with the NGS data. Interestingly, some isolates had two distinct plasmids encoding blaNDM-1 that could be readily identified with ODM. The coexistence of different plasmids carrying the same blaNDM-1 gene in a single isolate has rarely been reported, probably because of limitations in plasmid characterization techniques. Conclusions: The plasmids encoding the blaNDM-1 gene in this study cohort were diverse and may represent a similar picture in Vietnamese society. The study highlights important aspects of the usefulness of ODM for plasmid analysis.

Optical DNA mapping


bla NDM-1



Sriram Kesarimangalam

Chalmers, Biologi och bioteknik, Kemisk biologi

Elina Ekedahl

Chalmers, Biologi och bioteknik, Kemisk biologi

Ngoc Thi Bich Hoang

Vietnam National Children's Hospital

Tsegaye Sewunet

Karolinska Institutet

Björn Berglund

Linköpings universitet

Ludwig Lundberg

Linköpings universitet

Sveriges lantbruksuniversitet (SLU)

Shoeib Nematzadeh

Karolinska Institutet

Maud Nilsson

Linköpings universitet

Lennart E. Nilsson

Linköpings universitet

Ngai Kien Le

Vietnam National Children's Hospital

Tran Minh Dien

Vietnam National Children's Hospital

Håkan Hanberger

Linköpings universitet

Linus Olson

Karolinska Institutet

Mattias Larsson

Karolinska Institutet

Christian G. Giske

Karolinska Institutet

Karolinska universitetssjukhuset

Fredrik Westerlund

Chalmers, Biologi och bioteknik, Kemisk biologi

International Journal of Antimicrobial Agents

0924-8579 (ISSN)

Vol. 59 2 106496



Medicinsk genetik






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