Xylan-cellulose thin film platform for assessing xylanase activity
Journal article, 2022

Enzymatic degradation of plant polysaccharide networks is a complex process that involves disrupting an intimate assembly of cellulose and hemicelluloses in fibrous matrices. To mimic this assembly and to elucidate the efficiency of enzymatic degradation in a rapid way, models with physicochemical equivalence to natural systems are needed. Here, we employ xylan-coated cellulose thin films to monitor the hydrolyzing activity of an endo-1,4-β-xylanase. In situ surface plasmon resonance spectroscopy (SPRS) revealed a decrease in xylan areal mass ranging from 0.01 ± 0.02 to 0.52 ± 0.04 mg·m−2. The extent of digestion correlates to increasing xylanase concentration. In addition, ex situ determination of released monosaccharides revealed that incubation time was also a significant factor in degradation (P > 0.01). For both experiments, atomic force microscopy confirmed the removal of xylans from the cellulose thin films. We provide a new model platform that offers nanoscale sensitivity for investigating biopolymer interactions and their susceptibility to enzymatic hydrolysis.

Trimethylsilyl cellulose

Biopolymer

Thin films

Xylanase

Xylan

Author

Jana B. Schaubeder

Technische Universität Graz

Jonas Ravn

Chalmers, Biology and Biological Engineering, Industrial Biotechnology

Eliott Jean Quentin Orzan

Chalmers, Chemistry and Chemical Engineering, Applied Chemistry

João Heitor Colombelli Manfrão Netto

Chalmers, Biology and Biological Engineering, Industrial Biotechnology

Cecilia Geijer

Chalmers, Biology and Biological Engineering, Industrial Biotechnology

Tiina Nypelö

Wallenberg Wood Science Center (WWSC)

Chalmers, Chemistry and Chemical Engineering, Applied Chemistry

Stefan Spirk

Technische Universität Graz

Carbohydrate Polymers

0144-8617 (ISSN)

Vol. 294 119737

Subject Categories

Physical Chemistry

Polymer Technologies

Other Chemistry Topics

DOI

10.1016/j.carbpol.2022.119737

PubMed

35868741

More information

Latest update

7/25/2022