Engineering Bacillus subtilis for production of 3-hydroxypropanoic acid
Journal article, 2023

3-Hydroxypropionic acid (3-HP) is a valuable platform chemical that is used as a precursor for several higher value-added chemical products. There is an increased interest in development of cell factories as a means for the synthesis of 3-HP and various other platform chemicals. For more than a decade, concentrated effort has been invested by the scientific community towards developing bio-based approaches for the production of 3-HP using primarily Escherichia coli and Klebsiella pneumoniae as production hosts. These hosts however might not be optimal for applications in e.g., food industry due primarily to endotoxin production and the pathogenic origin of particularly the K. pneumoniae. We have previously demonstrated that the generally recognized as safe organism Bacillus subtilis can be engineered to produce 3-HP using glycerol, an abundant by-product of the biodiesel industry, as substrate. For commercial exploitation, there is a need to substantially increase the titer. In the present study, we optimized the bioprocess conditions and further engineered the B. subtilis 3-HP production strain. Thereby, using glycerol as substrate, we were able to improve 3-HP production in a 1-L bioreactor to a final titer of 22.9 g/L 3-HP.

biosynthesis

metabolic engineering

glycerol

synthetic biology

cell factory

3-hydroxypropanoic acid

Author

Abhroop Garg

Technical University of Denmark (DTU)

C. Jers

Technical University of Denmark (DTU)

Hee Jin Hwang

Ajou University

Chalmers, Life Sciences, Systems and Synthetic Biology

Aida Kalantari

Chalmers, Life Sciences, Systems and Synthetic Biology

Ildze Ventina

Technical University of Denmark (DTU)

Ivan Mijakovic

Chalmers, Life Sciences, Systems and Synthetic Biology

Technical University of Denmark (DTU)

Frontiers in Bioengineering and Biotechnology

2296-4185 (eISSN)

Vol. 11 1101232

Subject Categories

Production Engineering, Human Work Science and Ergonomics

Chemical Process Engineering

Biocatalysis and Enzyme Technology

DOI

10.3389/fbioe.2023.1101232

PubMed

36726744

More information

Latest update

2/13/2023