Protein cohabitation: long-term immunoglobulin G storage at room temperature
Journal article, 2023
Long-term functional storage of therapeutic proteins at room temperature has been an eternal challenge. Inspired by the cellular cooperativity of proteins, we have taken a step forward to address this challenge by cohabitating Immunoglobulin G (IgG1) with a food protein gelatin in the solid-state at room temperature. Interestingly, IgG1 remained functionally active for a record 14 months revealed from the western-blot assay. Further quantification by HP-LC analysis showed 100% structural integrity of IgG1 with no degradation in the gelatin matrix during this period. The developed formulation has a direct application in oral medical nutrition therapy to cure gastrointestinal microbial infections. Also the strategy provides a robust energy economic alternative to the protein engineering methods for long-term functional storage of therapeutic proteins at room temperature.
Author
Pankaj Bharmoria
Spanish National Research Council (CSIC)
Chalmers, Chemistry and Chemical Engineering, Applied Chemistry
University of Gothenburg
Saik Ann Ooi
University of Gothenburg
Andrea Cellini
University of Gothenburg
Daniel Tietze
University of Gothenburg
Michal Maj
Uppsala University
Kasper Moth-Poulsen
Chalmers, Chemistry and Chemical Engineering, Applied Chemistry
Spanish National Research Council (CSIC)
Polytechnic University of Catalonia
Catalan Institution for Research and Advanced Studies
Alesia A. Tietze
University of Gothenburg
Journal of Materials Chemistry B
2050750x (ISSN) 20507518 (eISSN)
Vol. 11 24 5400-5405NIRLAMS - NIR Light Harvesting in Artificial Protein-Lipid-Chromophores Coassembled Molecular System
European Commission (EC) (EC/H2020/844972), 2020-01-01 -- 2022-12-31.
Subject Categories (SSIF 2011)
Infectious Medicine
Public Health, Global Health, Social Medicine and Epidemiology
DOI
10.1039/d3tb00161j
PubMed
37294537