Protein cohabitation: long-term immunoglobulin G storage at room temperature
Journal article, 2023

Long-term functional storage of therapeutic proteins at room temperature has been an eternal challenge. Inspired by the cellular cooperativity of proteins, we have taken a step forward to address this challenge by cohabitating Immunoglobulin G (IgG1) with a food protein gelatin in the solid-state at room temperature. Interestingly, IgG1 remained functionally active for a record 14 months revealed from the western-blot assay. Further quantification by HP-LC analysis showed 100% structural integrity of IgG1 with no degradation in the gelatin matrix during this period. The developed formulation has a direct application in oral medical nutrition therapy to cure gastrointestinal microbial infections. Also the strategy provides a robust energy economic alternative to the protein engineering methods for long-term functional storage of therapeutic proteins at room temperature.

Author

Pankaj Bharmoria

Chalmers, Chemistry and Chemical Engineering, Applied Chemistry

University of Gothenburg

Institute of Material Science of Barcelona (ICMAB)

Saik Ann Ooi

University of Gothenburg

Andrea Cellini

University of Gothenburg

Daniel Tietze

University of Gothenburg

Michal Maj

Uppsala University

Kasper Moth-Poulsen

Chalmers, Chemistry and Chemical Engineering, Applied Chemistry

Institute of Material Science of Barcelona (ICMAB)

Catalan Institution for Research and Advanced Studies

Polytechnic University of Catalonia

Alesia A. Tietze

University of Gothenburg

Journal of Materials Chemistry B

2050750x (ISSN) 20507518 (eISSN)

Vol. 11 24 5400-5405

NIRLAMS - NIR Light Harvesting in Artificial Protein-Lipid-Chromophores Coassembled Molecular System

European Commission (EC) (EC/H2020/844972), 2020-01-01 -- 2022-12-31.

Subject Categories

Infectious Medicine

Public Health, Global Health, Social Medicine and Epidemiology

DOI

10.1039/d3tb00161j

PubMed

37294537

More information

Latest update

6/26/2023