Protein cohabitation: long-term immunoglobulin G storage at room temperature
Artikel i vetenskaplig tidskrift, 2023
Long-term functional storage of therapeutic proteins at room temperature has been an eternal challenge. Inspired by the cellular cooperativity of proteins, we have taken a step forward to address this challenge by cohabitating Immunoglobulin G (IgG1) with a food protein gelatin in the solid-state at room temperature. Interestingly, IgG1 remained functionally active for a record 14 months revealed from the western-blot assay. Further quantification by HP-LC analysis showed 100% structural integrity of IgG1 with no degradation in the gelatin matrix during this period. The developed formulation has a direct application in oral medical nutrition therapy to cure gastrointestinal microbial infections. Also the strategy provides a robust energy economic alternative to the protein engineering methods for long-term functional storage of therapeutic proteins at room temperature.
Författare
Pankaj Bharmoria
Consejo Superior de Investigaciones Científicas (CSIC)
Chalmers, Kemi och kemiteknik, Tillämpad kemi
Göteborgs universitet
Saik Ann Ooi
Göteborgs universitet
Andrea Cellini
Göteborgs universitet
Daniel Tietze
Göteborgs universitet
Michal Maj
Uppsala universitet
Kasper Moth-Poulsen
Chalmers, Kemi och kemiteknik, Tillämpad kemi
Consejo Superior de Investigaciones Científicas (CSIC)
Universitat Politecnica de Catalunya
Institucio Catalana de Recerca i Estudis Avancats
Alesia A. Tietze
Göteborgs universitet
Journal of Materials Chemistry B
2050750x (ISSN) 20507518 (eISSN)
Vol. 11 24 5400-5405NIRLAMS - NIR Light Harvesting in Artificial Protein-Lipid-Chromophores Coassembled Molecular System
Europeiska kommissionen (EU) (EC/H2020/844972), 2020-01-01 -- 2022-12-31.
Ämneskategorier (SSIF 2011)
Infektionsmedicin
Folkhälsovetenskap, global hälsa, socialmedicin och epidemiologi
DOI
10.1039/d3tb00161j
PubMed
37294537