Protein cohabitation: long-term immunoglobulin G storage at room temperature
Artikel i vetenskaplig tidskrift, 2023
Long-term functional storage of therapeutic proteins at room temperature has been an eternal challenge. Inspired by the cellular cooperativity of proteins, we have taken a step forward to address this challenge by cohabitating Immunoglobulin G (IgG1) with a food protein gelatin in the solid-state at room temperature. Interestingly, IgG1 remained functionally active for a record 14 months revealed from the western-blot assay. Further quantification by HP-LC analysis showed 100% structural integrity of IgG1 with no degradation in the gelatin matrix during this period. The developed formulation has a direct application in oral medical nutrition therapy to cure gastrointestinal microbial infections. Also the strategy provides a robust energy economic alternative to the protein engineering methods for long-term functional storage of therapeutic proteins at room temperature.
Författare
Pankaj Bharmoria
Chalmers, Kemi och kemiteknik, Tillämpad kemi
Göteborgs universitet
Institut de Ciència de Materials de Barcelona (ICMAB-CSIC)
Saik Ann Ooi
Göteborgs universitet
Andrea Cellini
Göteborgs universitet
Daniel Tietze
Göteborgs universitet
Michal Maj
Uppsala universitet
Kasper Moth-Poulsen
Chalmers, Kemi och kemiteknik, Tillämpad kemi
Institut de Ciència de Materials de Barcelona (ICMAB-CSIC)
Institucio Catalana de Recerca i Estudis Avancats
Universitat Politecnica de Catalunya
Alesia A. Tietze
Göteborgs universitet
Journal of Materials Chemistry B
2050750x (ISSN) 20507518 (eISSN)
Vol. 11 24 5400-5405NIRLAMS - NIR Light Harvesting in Artificial Protein-Lipid-Chromophores Coassembled Molecular System
Europeiska kommissionen (EU) (EC/H2020/844972), 2020-01-01 -- 2022-12-31.
Ämneskategorier
Infektionsmedicin
Folkhälsovetenskap, global hälsa, socialmedicin och epidemiologi
DOI
10.1039/d3tb00161j
PubMed
37294537