Fluorescence Microscopy of Nanochannel-Confined DNA
Book chapter, 2024
Stretching of DNA in nanoscale confinement allows for several important studies. The genetic contents of the DNA can be visualized on the single DNA molecule level, and the polymer physics of confined DNA and also DNA/protein and other DNA/DNA-binding molecule interactions can be explored. This chapter describes the basic steps to fabricate the nanostructures, perform the experiments, and analyze the data.
Nanochannels
Fluorescence
DNA
Single molecule
Author
Sriram Kesarimangalam
Chalmers, Life Sciences, Chemical Biology
Fredrik Persson
Moleculent
Joachim Fritzsche
Chalmers, Physics, Chemical Physics
Jason P. Beech
Lund University
J. O. Tegenfeldt
Lund University
Fredrik Westerlund
Chalmers, Life Sciences, Chemical Biology
Methods in Molecular Biology
10643745 (ISSN) 1940-6029 (eISSN)
Vol. 2694 175-202Genomic diagnostics beyond the sequence (BeyondSeq)
European Commission (EC) (EC/H2020/634890), 2015-06-01 -- 2019-12-31.
Next Generation Nanofluidic Devices for Single Molecule Analysis of DNA Repair Dynamics
European Commission (EC) (EC/H2020/866238), 2020-04-01 -- 2025-03-31.
Real-Time Visualization of DNA Repair - One Molecule at a Time
Swedish Research Council (VR) (2020-03400), 2021-01-01 -- 2024-12-31.
Subject Categories
Physical Chemistry
Biophysics
Condensed Matter Physics
DOI
10.1007/978-1-0716-3377-9_9
PubMed
37824005