Fluorescence Microscopy of Nanochannel-Confined DNA
Kapitel i bok, 2024
Stretching of DNA in nanoscale confinement allows for several important studies. The genetic contents of the DNA can be visualized on the single DNA molecule level, and the polymer physics of confined DNA and also DNA/protein and other DNA/DNA-binding molecule interactions can be explored. This chapter describes the basic steps to fabricate the nanostructures, perform the experiments, and analyze the data.
Nanochannels
Fluorescence
DNA
Single molecule
Författare
Sriram Kesarimangalam
Chalmers, Life sciences, Kemisk biologi
Fredrik Persson
Moleculent
Joachim Fritzsche
Chalmers, Fysik, Kemisk fysik
Jason P. Beech
Lunds universitet
J. O. Tegenfeldt
Lunds universitet
Fredrik Westerlund
Chalmers, Life sciences, Kemisk biologi
Methods in Molecular Biology
10643745 (ISSN) 1940-6029 (eISSN)
Vol. 2694 175-202Genomic diagnostics beyond the sequence (BeyondSeq)
Europeiska kommissionen (EU) (EC/H2020/634890), 2015-06-01 -- 2019-12-31.
Next Generation Nanofluidic Devices for Single Molecule Analysis of DNA Repair Dynamics
Europeiska kommissionen (EU) (EC/H2020/866238), 2020-04-01 -- 2025-03-31.
Visualisering av DNA-Reparation - En Molekyl i Taget
Vetenskapsrådet (VR) (2020-03400), 2021-01-01 -- 2024-12-31.
Ämneskategorier
Fysikalisk kemi
Biofysik
Den kondenserade materiens fysik
DOI
10.1007/978-1-0716-3377-9_9
PubMed
37824005