Aberrant microbiomes are associated with increased antibiotic resistance gene load in hybrid mice
Journal article, 2024
Antibiotic resistance is a priority public health problem resulting from eco-evolutionary dynamics within microbial communities and their interaction at a mammalian host interface or geographical scale. The links between mammalian host genetics, bacterial gut community, and antimicrobial resistance gene (ARG) content must be better understood in natural populations inhabiting heterogeneous environments. Hybridization, the interbreeding of genetically divergent populations, influences different components of the gut microbial communities. However, its impact on bacterial traits such as antibiotic resistance is unknown. Here, we present that hybridization might shape bacterial communities and ARG occurrence. We used amplicon sequencing to study the gut microbiome and to predict ARG composition in natural populations of house mice (Mus musculus). We compared gastrointestinal bacterial and ARG diversity, composition, and abundance across a gradient of pure and hybrid genotypes in the European House Mouse Hybrid Zone. We observed an increased overall predicted richness of ARG in hybrid mice. We found bacteria-ARG interactions by their co-abundance and detected phenotypes of extreme abundances in hybrid mice at the level of specific bacterial taxa and ARGs, mainly multidrug resistance genes. Our work suggests that mammalian host genetic variation impacts the gut microbiome and chromosomal ARGs. However, it raises further questions on how the mammalian host genetics impact ARGs via microbiome dynamics or environmental covariates.
hybridization
mice
antimicrobial resistance gene
microbiome
Author
Victor Hugo Jarquin-Diaz
Expt & Clin Res Ctr
Helmholtz Association of German Research Centres
Humboldt University of Berlin
Leibniz Inst Zoo & Wildlife Res IZW
Freie Universität Berlin
Charité University Medicine Berlin
Susana Carolina Martins Ferreira
Humboldt University of Berlin
University of Vienna
Alice Balard
Leibniz Inst Zoo & Wildlife Res IZW
Humboldt University of Berlin
Ludovit Dureje
Czech Academy of Sciences
Milos Macholan
Czech Academy of Sciences
Jaroslav Pialek
Czech Academy of Sciences
Johan Bengtsson Palme
University of Gothenburg
Chalmers, Life Sciences, Systems and Synthetic Biology
Stephanie Kramer-Schadt
Leibniz Inst Zoo & Wildlife Res IZW
Technische Universität Berlin
Sofia Kirke Forslund-Startceva
German Center for Cardiovascular Research DZHK
Charité University Medicine Berlin
Expt & Clin Res Ctr
Freie Universität Berlin
Humboldt University of Berlin
Helmholtz Association of German Research Centres
Emanuel Heitlinger
Humboldt University of Berlin
Leibniz Inst Zoo & Wildlife Res IZW
ISME COMMUNICATIONS
2730-6151 (eISSN)
Vol. 4 1 ycae053EMBARK: Establishing a Monitoring Baseline for Antibiotic Resistance in Key environments
Swedish Research Council (VR) (2019-00299), 2022-05-01 -- 2023-12-31.
Roots
Basic sciences
Areas of Advance
Health Engineering
Subject Categories (SSIF 2011)
Microbiology
Bioinformatics (Computational Biology)
Microbiology in the medical area
DOI
10.1093/ismeco/ycae053
PubMed
38800129