Aberrant microbiomes are associated with increased antibiotic resistance gene load in hybrid mice
Artikel i vetenskaplig tidskrift, 2024
Antibiotic resistance is a priority public health problem resulting from eco-evolutionary dynamics within microbial communities and their interaction at a mammalian host interface or geographical scale. The links between mammalian host genetics, bacterial gut community, and antimicrobial resistance gene (ARG) content must be better understood in natural populations inhabiting heterogeneous environments. Hybridization, the interbreeding of genetically divergent populations, influences different components of the gut microbial communities. However, its impact on bacterial traits such as antibiotic resistance is unknown. Here, we present that hybridization might shape bacterial communities and ARG occurrence. We used amplicon sequencing to study the gut microbiome and to predict ARG composition in natural populations of house mice (Mus musculus). We compared gastrointestinal bacterial and ARG diversity, composition, and abundance across a gradient of pure and hybrid genotypes in the European House Mouse Hybrid Zone. We observed an increased overall predicted richness of ARG in hybrid mice. We found bacteria-ARG interactions by their co-abundance and detected phenotypes of extreme abundances in hybrid mice at the level of specific bacterial taxa and ARGs, mainly multidrug resistance genes. Our work suggests that mammalian host genetic variation impacts the gut microbiome and chromosomal ARGs. However, it raises further questions on how the mammalian host genetics impact ARGs via microbiome dynamics or environmental covariates.
antimicrobial resistance gene
microbiome
mice
hybridization
Författare
Victor Hugo Jarquin-Diaz
Freie Universität Berlin
Charité Universitätsmedizin Berlin
Leibniz Inst Zoo & Wildlife Res IZW
Humboldt-Universität zu Berlin
Max Delbruck Ctr Mol Med Helmholtz Assoc MDC
Expt & Clin Res Ctr
Susana Carolina Martins Ferreira
Humboldt-Universität zu Berlin
Universität Wien
Alice Balard
Leibniz Inst Zoo & Wildlife Res IZW
Humboldt-Universität zu Berlin
Ludovit Dureje
Czech Academy of Sciences
Milos Macholan
Czech Academy of Sciences
Jaroslav Pialek
Czech Academy of Sciences
Johan Bengtsson Palme
Göteborgs universitet
Chalmers, Life sciences, Systembiologi
Stephanie Kramer-Schadt
Technische Universität Berlin
Leibniz Inst Zoo & Wildlife Res IZW
Sofia Kirke Forslund-Startceva
Humboldt-Universität zu Berlin
Deutsches Zentrum für Herz-Kreislauf-Forshung (DZHK)
Expt & Clin Res Ctr
Charité Universitätsmedizin Berlin
Freie Universität Berlin
Max Delbruck Ctr Mol Med Helmholtz Assoc MDC
Emanuel Heitlinger
Leibniz Inst Zoo & Wildlife Res IZW
Humboldt-Universität zu Berlin
ISME COMMUNICATIONS
2730-6151 (eISSN)
Vol. 4 1 ycae053Etablering av normalnivåer för antibiotikaresistens i olika miljöer för miljöövervakning
Vetenskapsrådet (VR) (2019-00299), 2022-05-01 -- 2023-12-31.
Ämneskategorier
Mikrobiologi
Bioinformatik (beräkningsbiologi)
Mikrobiologi inom det medicinska området
DOI
10.1093/ismeco/ycae053
PubMed
38800129