Aberrant microbiomes are associated with increased antibiotic resistance gene load in hybrid mice
Artikel i vetenskaplig tidskrift, 2024
Antibiotic resistance is a priority public health problem resulting from eco-evolutionary dynamics within microbial communities and their interaction at a mammalian host interface or geographical scale. The links between mammalian host genetics, bacterial gut community, and antimicrobial resistance gene (ARG) content must be better understood in natural populations inhabiting heterogeneous environments. Hybridization, the interbreeding of genetically divergent populations, influences different components of the gut microbial communities. However, its impact on bacterial traits such as antibiotic resistance is unknown. Here, we present that hybridization might shape bacterial communities and ARG occurrence. We used amplicon sequencing to study the gut microbiome and to predict ARG composition in natural populations of house mice (Mus musculus). We compared gastrointestinal bacterial and ARG diversity, composition, and abundance across a gradient of pure and hybrid genotypes in the European House Mouse Hybrid Zone. We observed an increased overall predicted richness of ARG in hybrid mice. We found bacteria-ARG interactions by their co-abundance and detected phenotypes of extreme abundances in hybrid mice at the level of specific bacterial taxa and ARGs, mainly multidrug resistance genes. Our work suggests that mammalian host genetic variation impacts the gut microbiome and chromosomal ARGs. However, it raises further questions on how the mammalian host genetics impact ARGs via microbiome dynamics or environmental covariates.
hybridization
antimicrobial resistance gene
mice
microbiome
Författare
Victor Hugo Jarquin-Diaz
Charité Universitätsmedizin Berlin
Expt & Clin Res Ctr
Helmholtz-Gemeinschaft Deutscher Forschungszentren
Humboldt-Universität zu Berlin
Freie Universität Berlin
Leibniz-Gemeinschaft
Susana Carolina Martins Ferreira
Humboldt-Universität zu Berlin
Universität Wien
Alice Balard
Humboldt-Universität zu Berlin
Leibniz-Gemeinschaft
Ludovit Dureje
Czech Academy of Sciences
Milos Macholan
Czech Academy of Sciences
Jaroslav Pialek
Czech Academy of Sciences
Johan Bengtsson Palme
Chalmers, Life sciences, Systembiologi
Göteborgs universitet
Stephanie Kramer-Schadt
Leibniz-Gemeinschaft
Technische Universität Berlin
Sofia Kirke Forslund-Startceva
Charité Universitätsmedizin Berlin
Humboldt-Universität zu Berlin
Freie Universität Berlin
Expt & Clin Res Ctr
Deutsches Zentrum für Herz-Kreislauf-Forshung (DZHK)
Helmholtz-Gemeinschaft Deutscher Forschungszentren
Emanuel Heitlinger
Leibniz-Gemeinschaft
Humboldt-Universität zu Berlin
Isme Communications
27306151 (eISSN)
Vol. 4 1 ycae053Etablering av normalnivåer för antibiotikaresistens i olika miljöer för miljöövervakning
Vetenskapsrådet (VR) (2019-00299), 2022-05-01 -- 2023-12-31.
Fundament
Grundläggande vetenskaper
Styrkeområden
Hälsa och teknik
Ämneskategorier (SSIF 2011)
Mikrobiologi
Bioinformatik (beräkningsbiologi)
Mikrobiologi inom det medicinska området
DOI
10.1093/ismeco/ycae053
PubMed
38800129