Cryogenic probe technology enables multidimensional solid-state NMR of the stratum corneum without isotope labeling
Journal article, 2024

Solid-state NMR has great potential for investigating molecular structure, dynamics, and organization of the stratum corneum, the outer 10–20 μm of the skin, but is hampered by the unfeasibility of isotope labelling as generally required to reach sufficient signal-to-noise ratio for the more informative multidimensional NMR techniques. In this preliminary study of pig stratum corneum at 35 °C and water-free conditions, we demonstrate that cryogenic probe technology offers sufficient signal boost to observe previously undetectable minor resonances that can be uniquely assigned to fluid cholesterol, ceramides, and triacylglycerols, as well as enables 1H–1H spin diffusion monitored by 2D 1H-13C HETCOR to estimate 1–100 nm distances between specific atomic sites on proteins and lipids. The new capabilities open up for future multidimensional solid-state NMR studies to answer long-standing questions about partitioning of additives, such as pharmaceutically active substances, between solid and liquid domains within the protein and lipid phases in the stratum corneum and the lipids of the sebum.

Author

Barbara Perrone

Bruker BioSpin

Maria Gunnarsson

Lund University

Diana Bernin

Chalmers, Chemistry and Chemical Engineering, Chemical Technology

Emma Sparr

Lund University

D. Topgaard

Lund University

Solid State Nuclear Magnetic Resonance

0926-2040 (ISSN) 1527-3326 (eISSN)

Vol. 134 101972

Subject Categories

Physical Chemistry

Organic Chemistry

DOI

10.1016/j.ssnmr.2024.101972

More information

Latest update

10/7/2024