Applying 3D cultures and high-throughput technologies to study host-pathogen interactions
Review article, 2025
Recent advances in cell culturing and DNA sequencing have dramatically altered the field of human microbiome research. Three-dimensional (3D) cell culture is an important tool in cell biology, in cancer research, and for studying host-microbe interactions, as it mimics the in vivo characteristics of the host environment in an in vitro system, providing reliable and reproducible models. This work provides an overview of the main 3D culture techniques applied to study interactions between host cells and pathogenic microorganisms, how these systems can be integrated with high-throughput molecular methods, and how multi-species model systems may pave the way forward to pinpoint interactions among host, beneficial microbes and pathogens.
organ-on-a-chip
tridimensional cell culture
high-throughput sequencing
microbial model communities
transposon sequencing (TnSeq)
organoids
rotating wall vessel (RWV)
Author
E. C.P. De Martinis
University of Sao Paulo (USP)
Virgínia Farias Alves
Federal University of Goiás
Marita Gimenez Pereira
University of Sao Paulo (USP)
Leonardo Neves Andrade
University of Sao Paulo (USP)
Nathalia Abichabki
Chalmers, Life Sciences, Systems and Synthetic Biology
University of Sao Paulo (USP)
Anna Abramova
Chalmers, Life Sciences, Systems and Synthetic Biology
University of Gothenburg
Mirjam Dannborg
University of Gothenburg
Chalmers, Life Sciences, Systems and Synthetic Biology
Johan Bengtsson Palme
University of Gothenburg
Chalmers, Life Sciences, Systems and Synthetic Biology
Frontiers in Immunology
1664-3224 (eISSN)
Vol. 16 1488699Predicting future pathogenicity and antibiotic resistance
Swedish Foundation for Strategic Research (SSF) (FFL21-0174), 2022-08-01 -- 2027-12-31.
Low-level Exposure to Antibiotics Disturbs Interactions among Non-pathogenic bacteria in the Gastrointestinal tract
Swedish Research Council (VR) (2020-03629), 2022-05-01 -- 2024-12-31.
Subject Categories (SSIF 2025)
Cell and Molecular Biology
Infectious Medicine
Microbiology in the Medical Area
DOI
10.3389/fimmu.2025.1488699