Applying 3D cultures and high-throughput technologies to study host-pathogen interactions
Reviewartikel, 2025
Recent advances in cell culturing and DNA sequencing have dramatically altered the field of human microbiome research. Three-dimensional (3D) cell culture is an important tool in cell biology, in cancer research, and for studying host-microbe interactions, as it mimics the in vivo characteristics of the host environment in an in vitro system, providing reliable and reproducible models. This work provides an overview of the main 3D culture techniques applied to study interactions between host cells and pathogenic microorganisms, how these systems can be integrated with high-throughput molecular methods, and how multi-species model systems may pave the way forward to pinpoint interactions among host, beneficial microbes and pathogens.
organ-on-a-chip
tridimensional cell culture
high-throughput sequencing
microbial model communities
transposon sequencing (TnSeq)
organoids
rotating wall vessel (RWV)
Författare
E. C.P. De Martinis
Universidade de Sao Paulo (USP)
Virgínia Farias Alves
Universidade Federal de Goias
Marita Gimenez Pereira
Universidade de Sao Paulo (USP)
Leonardo Neves Andrade
Universidade de Sao Paulo (USP)
Nathalia Abichabki
Chalmers, Life sciences, Systembiologi
Universidade de Sao Paulo (USP)
Anna Abramova
Chalmers, Life sciences, Systembiologi
Göteborgs universitet
Mirjam Dannborg
Göteborgs universitet
Chalmers, Life sciences, Systembiologi
Johan Bengtsson Palme
Göteborgs universitet
Chalmers, Life sciences, Systembiologi
Frontiers in Immunology
1664-3224 (eISSN)
Vol. 16 1488699Framtidens patogener och resistensgener
Stiftelsen för Strategisk forskning (SSF) (FFL21-0174), 2022-08-01 -- 2027-12-31.
Låga koncentrationer av antibiotika stör viktiga interaktioner mellan bakterier i det mänskliga mikrobiomet
Vetenskapsrådet (VR) (2020-03629), 2022-05-01 -- 2024-12-31.
Ämneskategorier (SSIF 2025)
Cell- och molekylärbiologi
Infektionsmedicin
Mikrobiologi inom det medicinska området
DOI
10.3389/fimmu.2025.1488699