Is there a correlation between airborne bacterial load measured by volumetric air sampling and fluorescent particle counting?

Journal article, 2026

Background Microbiological air sampling in operating rooms, based on colony-forming unit (CFU) counts, is time-consuming and costly. Novel technologies, such as particle counters that detect fluorescent bioparticles (FBPs), may offer a simpler method for estimating airborne bacterial loads. This study aimed to assess the correlation between CFU and FBP counts under realistic operating room conditions. Methods During 22 primary hip arthroplasty procedures at Uppsala university hospital, CFUs were measured using a Sartorius MD8 air sampler placed adjacent to the surgical field. Simultaneously, the Bio Aerosol Measuring System (BAMS), recorded FBPs sized 0.5-25 µm. FBP counts were analyzed during CFU sampling periods and using each CFU sampling interval as an individual data point, yielding 115 paired comparisons. Correlations were assessed using Spearman’s rank correlation coefficient. Results No significant correlation was observed between FBP and CFU counts. Comparing FBP with CFU counts during Sartorius sampling intervals, rho was −0.31 ( P = .23). Using individual CFU sampling intervals, the correlation for particles ≥ 3 µm rho was = −0.10 ( P = .35). Conclusions Analyses revealed no correlation between FBP and CFU counts in operation room air quality assessments, suggesting that BAMS-based FBP measurement is not a suitable alternative to established microbiological methods.