Cell-Based Assay To Quantify the Antioxidant Effect of Food-Derived Carotenoids Enriched in Postprandial Human Chylomicrons
Journal article, 2010

We developed a new method to evaluate the antioxidant effect of food products in a biological system. The antioxidant status of HepG2 cells was quantified after incubation with postprandial human chylomicrons after the intake of vegetable products. Three subjects consumed in a meal a vegetable soup containing 8.4 mg of beta-carotene and 9 mg of lycopene. After 5 h, the subjects consumed a second meal without carotenoids. Blood samples were collected at basal time and every hour for 9 h. Chylomicrons were isolated from serum samples and used for both carotenoid quantification and HepG2 stimulation. Carotenoid in chylomicrons followed an inter-individual and bimodal carotenoid response. We demonstrated the antioxidant effect of postprandial chylomicrons in HepG2 at the time of maximum carotenoid concentration of chylomicrons with respect to basal time. This cell-based assay seems to be a useful method to evaluate the antioxidant effect of fruit and vegetable products in a biological system.

HepG2 cells

dietary

beta-carotene

salmonella-typhimurium

Reactive oxygen species

tomato lycopene

postprandial

lycopene

remnants

hepg2 cells

chylomicron

beta-carotene

oxidative stress

vegetable and fruit food

metabolism

bioavailability

plasma

Author

M. Sanchez-Campillo

University of Murcia

F. Perez-Llamas

University of Murcia

D. Gonzalez-Silvera

University of Murcia

R. Martinez-Tomas

University of Murcia

M. I. Burgos

Hospital Virgen de la Arrixaca

Anna Wellner

Chalmers, Chemical and Biological Engineering, Life Sciences

F. Aviles

Hospital Virgen de la Arrixaca

S. Parra

Hospital Virgen de la Arrixaca

L Bialek

Unilever

Marie Alminger

Chalmers, Chemical and Biological Engineering, Life Sciences

E. Larque

University of Murcia

Journal of Agricultural and Food Chemistry

0021-8561 (ISSN) 1520-5118 (eISSN)

Vol. 58 20 10864-10868

Subject Categories

Food Science

Chemical Sciences

Areas of Advance

Life Science Engineering (2010-2018)

DOI

10.1021/jf102627g

More information

Latest update

3/1/2021 4