Nonlinear microscopy of lipid storage and fibrosis in muscle and liver tissues of mice fed high-fat diets
Journal article, 2010

Hallmarks of high-fat Western diet intake, such as excessive lipid accumulation in skeletal muscle and liver as well as liver fibrosis, are investigated in tissues from mice using nonlinear microscopy, second harmonic generation (SHG), and coherent anti-Stokes Raman scattering (CARS), supported by conventional analysis methods. Two aspects are presented; intake of standard chow versus Western diet, and a comparison between two high-fat Western diets of different polyunsaturated lipid content. CARS microscopy images of intramyocellular lipid droplets in muscle tissue show an increased amount for Western diet compared to standard diet samples. Even stronger diet impact is found for liver samples, where combined CARS and SHG microscopy visualize clear differences in lipid content and collagen fiber development, the latter indicating nonalcoholic fatty liver disease (NAFLD) and steatohepatitis induced at a relatively early stage for Western diet. Characteristic for NAFLD, the fibrous tissue-containing lipids accumulate in larger structures. This is also observed in CARS images of liver samples from two Western-type diets of different polyunsaturated lipid contents. In summary, nonlinear microscopy has strong potential (further promoted by technical advances toward clinical use) for detection and characterization of steatohepatitis already in its early stages.

harmonic generation

muscle

optical microscopy

coherent antiStokes Raman scattering

liver

diseases

lipid bilayers

cellular biophysics

Author

Christian Brackmann

Chalmers, Chemical and Biological Engineering, Molecular Imaging

Britt Gabrielsson

Chalmers, Chemical and Biological Engineering, Life Sciences, Food and Nutrition Science

Fredrik Svedberg

Chalmers, Chemical and Biological Engineering, Molecular Imaging

Agneta Holmäng

University of Gothenburg

Ann-Sofie Sandberg

Chalmers, Chemical and Biological Engineering, Life Sciences, Food and Nutrition Science

Annika Enejder

Chalmers, Chemical and Biological Engineering, Molecular Imaging

Journal of Biomedical Optics

1083-3668 (ISSN)

Vol. 15 6 066008-1 - 066008-10- 066008

Subject Categories

Endocrinology and Diabetes

Medical Laboratory and Measurements Technologies

Medical Genetics

Roots

Basic sciences

Areas of Advance

Life Science Engineering

DOI

10.1117/1.3505024

More information

Created

10/7/2017