Measuring absolute number concentrations of nanoparticles using single-particle tracking
Journal article, 2011

Single-particle tracking (SPT) microscopy is increasingly used to characterize nanoparticulate systems. We introduce a concept for estimation of particle number concentration in Brownian particle dispersions using SPT based on a model for the trajectory length distribution of particles to estimate the detection region volume. The resulting method is independent of precalibration reference measurements, and robust with respect to image processing settings. Experimentally estimated concentrations of different dilutions of 0.19- and 0.52-mu m polymer nanospheres are in excellent agreement with estimates computed from the concentrations of the stock solutions.




Magnus Röding

SuMo Biomaterials

Chalmers, Mathematical Sciences, Mathematical Statistics

University of Gothenburg

Hendrik Deschout

Kevin Braeckmans

Mats Rudemo

University of Gothenburg

Chalmers, Mathematical Sciences, Mathematical Statistics

Physical Review E

24700045 (ISSN) 24700053 (eISSN)

Vol. 84 3

Subject Categories

Physical Sciences



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