High-Resolution Micropatterned Teflon AF Substrates for Biocompatible Nanofluidic Devices
Journal article, 2012

We describe a general photolithography-based process for the microfabrication of surface-supported Teflon AF structures. Teflon AF patterns primarily benefit from superior optical properties such as very low autofluorescence and a low refractive index. The process ensures that the Teflon AF patterns remain strongly hydrophobic in order to allow rapid lipid monolayer spreading and generates a characteristic edge morphology which assists directed cell growth along the structured surfaces. We provide application examples, demonstrating the well-controlled mixing of lipid films on Teflon AF structures and showing how the patterned surfaces can be used as biocompatible growth-directing substrates for cell culture. Chinese hamster ovary (CHO) cells develop in a guided fashion along the sides of the microstructures, selectively avoiding to grow over the patterned areas.

films

surfaces

lipid-bilayers

membranes

growth

planar

cells

area

adhesion

Author

Ilja Czolkos

Chalmers, Chemical and Biological Engineering, Physical Chemistry

Bodil Hakonen

Chalmers, Chemical and Biological Engineering, Nuclear Chemistry

Owe Orwar

Chalmers, Chemical and Biological Engineering, Physical Chemistry

Aldo Jesorka

Chalmers, Chemical and Biological Engineering, Physical Chemistry

Langmuir

07437463 (ISSN) 15205827 (eISSN)

Vol. 28 6 3200-3205

Subject Categories

Chemical Sciences

DOI

10.1021/la2044784

More information

Created

10/8/2017