Hemocompatibility of siRNA loaded dextran nanogels
Journal article, 2011
Although the behavior of nanoscopic delivery systems in blood is an important parameter when contemplating their intravenous injection, this aspect is often poorly investigated when advancing from in vitro to in vivo experiments. In this paper, the behavior of siRNA loaded dextran nanogels in human plasma and blood is examined using fluorescence fluctuation spectroscopy, platelet aggregometry, flow cytometry and single particle tracking. Our results show that, in contrast to their negatively charged counterparts, positively charged siRNA loaded dextran nanogels cause platelet aggregation and show increased binding to human blood cells. Although PEGylating the nanogels did not have a significant effect on their interaction with blood cells, single particle tracking revealed that it is necessary to prevent their aggregation in human plasma. We therefore conclude that PEGylated negatively charged dextran nanogels are the most suited for further in vivo studies as they do not aggregate in human plasma and exhibit minimal interactions with blood cells.
gene therapy
blood compatibility
haemocompatibility
blood
drug delivery
nanoparticle
Author
B Naeye
Ghent university
H Deschout
Ghent university
Magnus Röding
Chalmers, Mathematical Sciences, Mathematical Statistics
SuMo Biomaterials
University of Gothenburg
Mats Rudemo
Chalmers, Mathematical Sciences, Mathematical Statistics
University of Gothenburg
J Delanghe
Center for Medical Genetics
K Devreese
Center for Medical Genetics
J Demeester
Ghent university
K Braeckmans
Ghent university
SC De Smedt
Ghent university
K Raemdonck
Ghent university
Biomaterials
0142-9612 (ISSN) 18785905 (eISSN)
Vol. 32 34 9120-9127Subject Categories
Biomaterials Science
DOI
10.1016/j.biomaterials.2011.08.015
PubMed
21890194