Probing Physical Properties of a DNA- Protein Complex Using Nanofluidic Channels
Journal article, 2014
A method to investigate physical properties of a DNA-protein complex in solution is demonstrated. By using tapered nanochannels and lipid passivation the persistence length of a RecA filament formed on double-stranded DNA is determined to 1.15 μm, in agreement with the literature, without attaching protein or DNA to any handles or surfaces. © 2014 WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim.
Author
Karolin Frykholm
Chalmers, Chemical and Biological Engineering, Physical Chemistry
Mohammadreza Alizadehheidari
Chalmers, Chemical and Biological Engineering, Physical Chemistry
Joachim Fritzsche
Chalmers, Applied Physics, Chemical Physics
Jens Wigenius
Chalmers, Chemical and Biological Engineering, Physical Chemistry
M. Modesti
Inserm Transfert
Institut Paoli-Calmettes
Centre national de la recherche scientifique (CNRS)
F. Persson
Inserm Transfert
Uppsala University
Fredrik Westerlund
Chemical Biology
Small
1613-6810 (ISSN) 1613-6829 (eISSN)
Vol. 10 5 884-887Areas of Advance
Nanoscience and Nanotechnology
Life Science Engineering (2010-2018)
Subject Categories
Biophysics
DOI
10.1002/smll.201302028