Visualizing a protein quake with time-resolved X-ray scattering at a free-electron laser
Journal article, 2014

We describe a method to measure ultrafast protein structural changes using time-resolved wide-angle X-ray scattering at an X-ray free-electron laser. We demonstrated this approach using multiphoton excitation of the Blastochloris viridis photosynthetic reaction center, observing an ultrafast global conformational change that arises within picoseconds and precedes the propagation of heat through the protein. This provides direct structural evidence for a 'protein quake': the hypothesis that proteins rapidly dissipate energy through quake-like structural motions.

Biochemical Research Methods




David Arnlund

University of Gothenburg

Linda C Johansson

University of Gothenburg

Cecilia Wickstrand

University of Gothenburg

A. Barty

G. J. Williams

Erik Malmerberg

University of Gothenburg

J Davidsson

D. Milathianaki

D. P. Deponte

R. L. Shoeman

D. J. Wang

D. James

Gergely Katona

University of Gothenburg

Sebastian Westenhoff

University of Gothenburg

T. A. White

A. Aquila

S. Bari

Peter Berntsen

University of Gothenburg

M. Bogan

T. B. van Driel

R. B. Doak

K. S. Kjaer

M. Frank

R. Fromme

I. Grotjohann

R. Henning

M. S. Hunter

R. A. Kirian

I. Kosheleva

C. Kupitz

M. N. Liang

A. V. Martin

M. M. Nielsen

M. Messerschmidt

M. M. Seibert

Jennie Sjöhamn

University of Gothenburg

F. Stellato

U. Weierstall

N. A. Zatsepin

J. C. H. Spence

P. Fromme

I. Schlichting

S. Boutet

G. Groenhof

H. N. Chapman

Richard Neutze

University of Gothenburg

Nature Methods

1548-7091 (ISSN) 1548-7105 (eISSN)

Vol. 11 9 923-926

Subject Categories

Chemical Sciences



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