Visualizing a protein quake with time-resolved X-ray scattering at a free-electron laser
Artikel i vetenskaplig tidskrift, 2014
We describe a method to measure ultrafast protein structural changes using time-resolved wide-angle X-ray scattering at an X-ray free-electron laser. We demonstrated this approach using multiphoton excitation of the Blastochloris viridis photosynthetic reaction center, observing an ultrafast global conformational change that arises within picoseconds and precedes the propagation of heat through the protein. This provides direct structural evidence for a 'protein quake': the hypothesis that proteins rapidly dissipate energy through quake-like structural motions.
Biochemical Research Methods
FREQUENCY VIBRATIONAL-MODES
STRUCTURAL DYNAMICS
Författare
David Arnlund
Göteborgs universitet
Linda C Johansson
Göteborgs universitet
Cecilia Wickstrand
Göteborgs universitet
A. Barty
G. J. Williams
Erik Malmerberg
Göteborgs universitet
J Davidsson
D. Milathianaki
D. P. Deponte
R. L. Shoeman
D. J. Wang
D. James
Gergely Katona
Göteborgs universitet
Sebastian Westenhoff
Göteborgs universitet
T. A. White
A. Aquila
S. Bari
Peter Berntsen
Göteborgs universitet
M. Bogan
T. B. van Driel
R. B. Doak
K. S. Kjaer
M. Frank
R. Fromme
I. Grotjohann
R. Henning
M. S. Hunter
R. A. Kirian
I. Kosheleva
C. Kupitz
M. N. Liang
A. V. Martin
M. M. Nielsen
M. Messerschmidt
M. M. Seibert
Jennie Sjöhamn
Göteborgs universitet
F. Stellato
U. Weierstall
N. A. Zatsepin
J. C. H. Spence
P. Fromme
I. Schlichting
S. Boutet
G. Groenhof
H. N. Chapman
Richard Neutze
Göteborgs universitet
Nature Methods
1548-7091 (ISSN) 1548-7105 (eISSN)
Vol. 11 9 923-926Ämneskategorier
Kemi
DOI
10.1038/nmeth.3067