The impact of respiration and oxidative stress response on recombinant ?-amylase production by Saccharomyces cerevisiae
Journal article, 2016

Studying protein production is important for fundamental research on cell biology and applied research for biotechnology. Yeast Saccharomyces cerevisiae is an attractive workhorse for production of recombinant proteins as it does not secrete many endogenous proteins and it is therefore easy to purify a secreted product. However, recombinant production at high rates represents a significant metabolic burden for the yeast cells, which results in oxidative stress and ultimately affects the protein production capacity. Here we describe a method to reduce the overall oxidative stress by overexpressing the endogenous HAP1 gene in a S. cerevisiae strain overproducing recombinant α-amylase. We demonstrate how Hap1p can activate a set of oxidative stress response genes and meanwhile contribute to increase the metabolic rate of the yeast strains, therefore mitigating the negative effect of the ROS accumulation associated to protein folding and hence increasing the production capacity during batch fermentations.

Amylase

Hap1

Oxidative stress response

Protein production

Yeast

Author

Jose Luis Martinez Ruiz

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

Eugenio Meza

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

Dina Petranovic Nielsen

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

Jens B Nielsen

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

Metabolic Engineering Communications

2214-0301 (eISSN)

Vol. 3 205-210

Areas of Advance

Energy

Life Science Engineering

Subject Categories

Bioinformatics and Systems Biology

DOI

10.1016/j.meteno.2016.06.003

More information

Created

10/8/2017