Light-sensing via hydrogen peroxide and a peroxiredoxin
Journal article, 2017

Yeast lacks dedicated photoreceptors; however, blue light still causes pronounced oscillations of the transcription factor Msn2 into and out of the nucleus. Here we show that this poorly understood phenomenon is initiated by a peroxisomal oxidase, which converts light into a hydrogen peroxide (H2O2) signal that is sensed by the peroxiredoxin Tsa1 and transduced to thioredoxin, to counteract PKA-dependent Msn2 phosphorylation. Upon H2O2, the nuclear retention of PKA catalytic subunits, which contributes to delayed Msn2 nuclear concentration, is antagonized in a Tsa1-dependent manner. Conversely, peroxiredoxin hyperoxidation interrupts the H2O2 signal and drives Msn2 oscillations by superimposing on PKA feedback regulation. Our data identify a mechanism by which light could be sensed in all cells lacking dedicated photoreceptors. In particular, the use of H2O2 as a second messenger in signalling is common to Msn2 oscillations and to light-induced entrainment of circadian rhythms and suggests conserved roles for peroxiredoxins in endogenous rhythms.

yeast transcription factor

protein-kinase

core component

nuclear-localization

factor msn2p

glucose starvation

circadian-rhythms

saccharomyces-cerevisiae

life-span

gene disruption

Author

Kristofer Bodvard

Chalmers, Physics, Bionanophotonics

K. Peeters

University of Gothenburg

F. Roger

University of Gothenburg

N. Romanov

European Molecular Biology Laboratory

University of Vienna

A. Igbaria

University of Paris-Sud

Niek Welkenhuysen

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

G. Palais

University of Paris-Sud

W. Reiter

University of Vienna

M. B. Toledano

University of Paris-Sud

Mikael Käll

Chalmers, Physics, Bionanophotonics

M. Molin

University of Gothenburg

Nature Communications

2041-1723 (ISSN)

Vol. 8 14791

Subject Categories

Cell Biology

Biochemistry and Molecular Biology

Cell and Molecular Biology

DOI

10.1038/ncomms14791

PubMed

28337980

More information

Latest update

8/1/2018 1