Exploring the potential of Saccharomyces cerevisiae for biopharmaceutical protein production
Journal article, 2017

Production of recombinant proteins by yeast plays a vital role in the biopharmaceutical industry. It is therefore desirable to develop yeast platform strains for over-production of various biopharmaceutical proteins, but this requires fundamental knowledge of the cellular machinery, especially the protein secretory pathway. Integrated analyses of multi-omics datasets can provide comprehensive understanding of cellular function, and can enable systems biology-driven and mathematical model-guided strain engineering. Rational engineering and introduction of trackable genetic modifications using synthetic biology tools, coupled with high-throughput screening are, however, also efficient approaches to relieve bottlenecks hindering high-level protein production. Here we review advances in systems biology and metabolic engineering of yeast for improving recombinant protein production.

Machinery

Metabolic engineering

Throughput

Functions

Yeast

Recombinant proteins

Biology

Author

Guokun Wang

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

Mingtao Huang

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

Jens B Nielsen

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

Current Opinion in Biotechnology

0958-1669 (ISSN)

Vol. 48 77-84

Subject Categories

Biochemistry and Molecular Biology

Software Engineering

Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)

DOI

10.1016/j.copbio.2017.03.017

More information

Created

10/7/2017