Membrane Deformation Induces Clustering of Norovirus Bound to Glycosphingolipids in a Supported Cell-Membrane Mimic
Journal article, 2018

Quartz crystal microbalance with dissipation monitoring and total internal reflection fluorescence microscopy have been used to investigate binding of norovirus-like particles (noroVLPs) to a supported (phospho)lipid bilayer (SLB) containing a few percent of H or B type 1 glycosphingolipid (GSL) receptors. Although neither of these GSLs spontaneously form domains, noroVLPs were observed to form micron-sized clusters containing typically up to about 30 VLP copies, especially for B type 1, which is a higher-affinity receptor. This novel finding is explained by proposing a model implying that VLP-induced membrane deformation promotes VLP clustering, a hypothesis that was further supported by observing that functionalized gold nanoparticles were able to locally induce SLB deformation. Because similar effects are likely possible also at cellular membranes, our findings are interesting beyond a pure biophysicochemical perspective as they shed new light on what may happen during receptor-mediated uptake of viruses as well as nanocarriers in drug delivery.

Author

Nagma Parveen

KU Leuven

Chalmers, Physics, Biological Physics

Inga Rimkute

Sahlgrenska Academy

Stephan Block

Chalmers, Physics, Biological Physics

Freie Universität Berlin

Gustaf E Rydell

Sahlgrenska Academy

Daniel Midtvedt

Chalmers, Physics, Biological Physics

G. Larson

Sahlgrenska Academy

V. P. Hytonen

University of Tampere

Vladimir Zhdanov

Russian Academy of Sciences

Chalmers, Physics, Chemical Physics

Anders Lundgren

Chalmers, Physics, Biological Physics

Fredrik Höök

University of Gothenburg

Chalmers, Physics, Biological Physics

Journal of Physical Chemistry Letters

1948-7185 (eISSN)

Vol. 9 9 2278-2284

Live dynamics of bacterial fimbriae and the formation of pathogenic biofilms

Swedish Research Council (VR), 2014-01-01 -- 2016-05-31.

Surface-sensitive microscopy for studies of cellular membranes

Swedish Research Council (VR), 2015-01-01 -- 2018-12-31.

Subject Categories

Pharmaceutical Sciences

Pharmacology and Toxicology

Biophysics

DOI

10.1021/acs.jpclett.8b00433

More information

Latest update

7/3/2018 2