Untargeted 1H NMR-Based Metabolomics Analysis of Urine and Serum Profiles after Consumption of Lentils, Chickpeas, and Beans: An Extended Meal Study to Discover Dietary Biomarkers of Pulses
Journal article, 2018

High legume intake has been shown to have beneficial effects on the health of humans. The use of nutritional biomarkers, as a complement to self-reported questionnaires, could assist in evaluating dietary intake and downstream effects on human health. The aim of this study was to investigate potential biomarkers of the consumption of pulses (i.e., white beans, chickpeas, and lentils) by using untargeted NMR-based metabolomics. Meals rich in pulses were consumed by a total of 11 participants in a randomized crossover study and multilevel partial least-squares regression was employed for paired comparisons. Metabolomics analysis indicated that trigonelline, 3-methylhistidine, dimethylglycine, trimethylamine, and lysine were potential, though not highly specific, biomarkers of pulse intake. Furthermore, monitoring of these metabolites for a period of 48 h after intake revealed a range of different excretion patterns among pulses. Following the consumption of pulses, a metabolomic profiling revealed that the concentration ratios of trigonelline, choline, lysine, and histidine were similar to those found in urine. In conclusion, this study identified potential urinary biomarkers of exposure to dietary pulses and provided valuable information about the time-response effect of these putative biomarkers.

legumes

NMR

dietary pulses

biomarkers

metabolomics

Author

Francisco Madrid-Gambin

University of Barcelona

Instituto de Salud Carlos III

Carl Brunius

Chalmers, Biology and Biological Engineering, Food and Nutrition Science

Mar Garcia-Aloy

Instituto de Salud Carlos III

University of Barcelona

Sheila Estruel-Amades

University of Barcelona

Rikard Landberg

Chalmers, Biology and Biological Engineering, Food and Nutrition Science

Swedish University of Agricultural Sciences (SLU)

Cristina Andres-Lacueva

University of Barcelona

Instituto de Salud Carlos III

Journal of Agricultural and Food Chemistry

0021-8561 (ISSN) 1520-5118 (eISSN)

Vol. 66 27 6997-7005

Optimal kost utifrån metabotyp för hälsa och vällevnad

Formas, 2016-01-01 -- 2021-12-31.

Subject Categories

Environmental Health and Occupational Health

Public Health, Global Health, Social Medicine and Epidemiology

Nutrition and Dietetics

DOI

10.1021/acs.jafc.8b00047

More information

Latest update

9/19/2018