Dissimilar Deformation of Fluid- and Gel-Phase Liposomes upon Multivalent Interaction with Cell Membrane Mimics Revealed Using Dual-Wavelength Surface Plasmon Resonance
Journal article, 2022

The mechanical properties of biological nanoparticles play a crucial role in their interaction with the cellular membrane, in particular for cellular uptake. This has significant implications for the design of pharmaceutical carrier particles. In this context, liposomes have become increasingly popular, among other reasons due to their customizability and easily varied physicochemical properties. With currently available methods, it is, however, not trivial to characterize the mechanical properties of nanoscopic liposomes especially with respect to the level of deformation induced upon their ligand-receptor-mediated interaction with laterally fluid cellular membranes. Here, we utilize the sensitivity of dual-wavelength surface plasmon resonance to probe the size and shape of bound liposomes (∼100 nm in diameter) as a means to quantify receptor-induced deformation during their interaction with a supported cell membrane mimic. By comparing biotinylated liposomes in gel and fluid phases, we demonstrate that fluid-phase liposomes are more prone to deformation than their gel-phase counterparts upon binding to the cell membrane mimic and that, as expected, the degree of deformation depends on the number of ligand-receptor pairs that are engaged in the multivalent binding.


Karin Norling

Chalmers, Biology and Biological Engineering, Chemical Biology

Mattias Sjöberg

Chalmers, Physics, Nano and Biophysics

Marta Bally

Umeå University

Vladimir Zhdanov

Russian Academy of Sciences

Chalmers, Physics

Nagma Parveen

Chalmers, Physics, Biological Physics

Fredrik Höök

Chalmers, Physics, Nano and Biophysics


07437463 (ISSN) 15205827 (eISSN)

Vol. 38 8 2550-2560

Two-dimensional flow nanometry for single nanoparticle analytics

Swedish Research Council (VR) (2018-04900), 2018-12-01 -- 2021-12-31.

Funktionell leverans av nukleotid-baserade läkemedel

Swedish Foundation for Strategic Research (SSF) (IRC15-0065), 2017-03-01 -- 2024-12-31.

Subject Categories

Biochemistry and Molecular Biology

Other Basic Medicine



Chalmers Materials Analysis Laboratory





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