Fluorescence Microscopy of Nanochannel-Confined DNA
Book chapter, 2024

Stretching of DNA in nanoscale confinement allows for several important studies. The genetic contents of the DNA can be visualized on the single DNA molecule level, and the polymer physics of confined DNA and also DNA/protein and other DNA/DNA-binding molecule interactions can be explored. This chapter describes the basic steps to fabricate the nanostructures, perform the experiments, and analyze the data.

Nanochannels

Fluorescence

DNA

Single molecule

Author

Sriram Kesarimangalam

Chalmers, Life Sciences, Chemical Biology

Fredrik Persson

Moleculent

Joachim Fritzsche

Chalmers, Physics, Chemical Physics

Jason P. Beech

Lund University

J. O. Tegenfeldt

Lund University

Fredrik Westerlund

Chalmers, Life Sciences, Chemical Biology

Methods in Molecular Biology

10643745 (ISSN) 1940-6029 (eISSN)

Vol. 2694 175-202

Genomic diagnostics beyond the sequence (BeyondSeq)

European Commission (EC) (EC/H2020/634890), 2015-06-01 -- 2019-12-31.

Next Generation Nanofluidic Devices for Single Molecule Analysis of DNA Repair Dynamics

European Commission (EC) (EC/H2020/866238), 2020-04-01 -- 2025-03-31.

Real-Time Visualization of DNA Repair - One Molecule at a Time

Swedish Research Council (VR) (2020-03400), 2021-01-01 -- 2024-12-31.

Subject Categories

Physical Chemistry

Biophysics

Condensed Matter Physics

DOI

10.1007/978-1-0716-3377-9_9

PubMed

37824005

More information

Latest update

7/12/2024