Fluorescence Microscopy of Nanochannel-Confined DNA
Kapitel i bok, 2024

Stretching of DNA in nanoscale confinement allows for several important studies. The genetic contents of the DNA can be visualized on the single DNA molecule level, and the polymer physics of confined DNA and also DNA/protein and other DNA/DNA-binding molecule interactions can be explored. This chapter describes the basic steps to fabricate the nanostructures, perform the experiments, and analyze the data.

Nanochannels

Fluorescence

DNA

Single molecule

Författare

Sriram Kesarimangalam

Chalmers, Life sciences, Kemisk biologi

Fredrik Persson

Moleculent

Joachim Fritzsche

Chalmers, Fysik, Kemisk fysik

Jason P. Beech

Lunds universitet

J. O. Tegenfeldt

Lunds universitet

Fredrik Westerlund

Chalmers, Life sciences, Kemisk biologi

Methods in Molecular Biology

10643745 (ISSN) 1940-6029 (eISSN)

Vol. 2694 175-202

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Europeiska kommissionen (EU) (EC/H2020/634890), 2015-06-01 -- 2019-12-31.

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Ämneskategorier

Fysikalisk kemi

Biofysik

Den kondenserade materiens fysik

DOI

10.1007/978-1-0716-3377-9_9

PubMed

37824005

Mer information

Senast uppdaterat

2024-07-12