Distribution of cholesterol and galactosylceramide in rat cerebellar white matter.
Journal article, 2006
White matter and the inner granular layer of rat cerebellum was analysed by imaging time-of-flight secondary-ion mass spectrometry (TOF-SIMS) equipped with a Bi+ ion cluster gun. Samples were prepared by high pressure freezing, freeze-fracturing and freeze drying or by plunge freezing and cryostat sectioning. The identified and localized chemical species were: sodium, potassium, phosphocholine, cholesterol and galactosylceramide (GalC) with carbon chain lengths C18:0 (N-stearoyl-galactosylceramide) and C24:0 (N-lignoceroylgalactosylceramide) with CH24:0 (hydroxy-lignoceroylgalactosylceramide). We report new findings regarding the organization of myelin in white matter. One is cholesterol-rich, ribbon-shaped 10-20 microm areas excluding Na+ and K+. The second finding is the different distribution of GalC C18 and GalC C24 in relation to these areas, where GalC C18 was localized in cholesterol-rich areas and GalC C24 was localized in Na/K-enriched areas. The distribution of GalC was in small spots, homogeneous in size, of 0.8-1.5 microm. Sample preparation with high pressure freezing allowed separate localization of sodium and potassium in tissue samples.
Myelin Sheath
metabolism
Male
chemistry
chemistry
Rats
Rats
Mass Spectrometry
metabolism
metabolism
Animals
chemistry
Cholesterol
chemistry
anatomy & histology
Cerebellum
Sprague-Dawley
Galactosylceramides
Author
Katrin Börner
University of Gothenburg
Håkan Nygren
University of Gothenburg
Birgit Hagenhoff
Per Malmberg
University of Gothenburg
Elke Tallarek
Jan-Eric Månsson
University of Gothenburg
Biochimica et biophysica acta
0006-3002 (ISSN)
Vol. 1761 3 335-44Subject Categories
MEDICAL AND HEALTH SCIENCES
DOI
10.1016/j.bbalip.2006.02.021
PubMed
16600673