Distribution of cholesterol and galactosylceramide in rat cerebellar white matter.
Artikel i vetenskaplig tidskrift, 2006

White matter and the inner granular layer of rat cerebellum was analysed by imaging time-of-flight secondary-ion mass spectrometry (TOF-SIMS) equipped with a Bi+ ion cluster gun. Samples were prepared by high pressure freezing, freeze-fracturing and freeze drying or by plunge freezing and cryostat sectioning. The identified and localized chemical species were: sodium, potassium, phosphocholine, cholesterol and galactosylceramide (GalC) with carbon chain lengths C18:0 (N-stearoyl-galactosylceramide) and C24:0 (N-lignoceroylgalactosylceramide) with CH24:0 (hydroxy-lignoceroylgalactosylceramide). We report new findings regarding the organization of myelin in white matter. One is cholesterol-rich, ribbon-shaped 10-20 microm areas excluding Na+ and K+. The second finding is the different distribution of GalC C18 and GalC C24 in relation to these areas, where GalC C18 was localized in cholesterol-rich areas and GalC C24 was localized in Na/K-enriched areas. The distribution of GalC was in small spots, homogeneous in size, of 0.8-1.5 microm. Sample preparation with high pressure freezing allowed separate localization of sodium and potassium in tissue samples.

Myelin Sheath

metabolism

Male

chemistry

chemistry

Rats

Rats

Mass Spectrometry

metabolism

metabolism

Animals

chemistry

Cholesterol

chemistry

anatomy & histology

Cerebellum

Sprague-Dawley

Galactosylceramides

Författare

Katrin Börner

Göteborgs universitet

Håkan Nygren

Göteborgs universitet

Birgit Hagenhoff

Per Malmberg

Göteborgs universitet

Elke Tallarek

Jan-Eric Månsson

Göteborgs universitet

Biochimica et biophysica acta

0006-3002 (ISSN)

Vol. 1761 3 335-44

Ämneskategorier

MEDICIN OCH HÄLSOVETENSKAP

DOI

10.1016/j.bbalip.2006.02.021

PubMed

16600673

Mer information

Skapat

2017-10-10