Single Molecule Imaging för att upptäcka DNA-skador i bakterier orsakas av antibiotika
Forskningsprojekt, 2022
– 2024
In this porject, we are working on establishing a method to detect different types of DNA damage in bacterial cells using single-molecule DNA microscopy. This method allows detecting individual DNA damage sites on a single DNA molecule. While general bacterial DNA damage reporters exist, most methods are indirect and determining the specific type of DNA damage has so far not been possible. We aim to overcome these limitations and develop a method that can be used on both bacterial cells and on isolated bacterial DNA, allowing distinction between direct and secondary effects. This is a considerable advantage over the current state-of-the-art.
The end goal of theproject is to detect DNA damage induced by antibiotics. DNA-damaging agents are typically discarded from antibiotic development for their expected toxicity, but certain compound classes do target DNA and are selective for bacterial cells. Yet, virtually nothing is known about how such compounds damage DNA. In light of the current antibiotic resistance crisis, it is pivotal not do prematurely discard drug candidates, so a re-evaluation of DNA-targeting compounds as antibacterial agents is in order. Our new method could help greatly with that.
Deltagare
Michaela Wenzel (kontakt)
Chalmers, Life sciences, Kemisk biologi
Obed Akwasi Aning
Chalmers, Life sciences, Kemisk biologi
Johanna Carlson
Chalmers, Life sciences, Kemisk biologi
Ann-Britt Schäfer
Chalmers, Life sciences, Kemisk biologi
Margareth Sidarta
Chalmers, Life sciences, Kemisk biologi
Fredrik Westerlund
Chalmers, Life sciences, Kemisk biologi
Finansiering
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Project Id: 11980
Ett fel har uppstått
Kontakta en administratör och delge följande feldata:
'Newtonsoft.Json.Linq.JValue' does not contain a definition for 'ContractOrganization'
at CallSite.Target(Closure, CallSite, Object) at System.Dynamic.UpdateDelegates.UpdateAndExecute1[T0,TRet](CallSite site, T0 arg0) at CallSite.Target(Closure, CallSite, Object) at ResearchWeb2024.Pages.Pages_Project.ExecuteAsync() in C:\home\site\repository\ResearchWeb2024\Pages\Project.cshtml:line 634
Project Id: 11980