Insight into factors directing high production of eukaryotic membrane proteins; production of 13 human AQPs in Pichia pastoris.
Artikel i vetenskaplig tidskrift, 2009

Membrane proteins are key players in all living cells. To achieve a better understanding of membrane protein function, significant amounts of purified protein are required for functional and structural analyses. Overproduction of eukaryotic membrane proteins, in particular, is thus an essential yet non-trivial task. Hence, improved understanding of factors which direct a high production of eukaryotic membrane proteins is desirable. In this study we have compared the overproduction of all human aquaporins in the eukaryotic host Pichia pastoris. We report quantitated production levels of each homologue and the extent of their membrane localization. Our results show that the protein production levels vary substantially, even between highly homologous aquaporins. A correlation between the extents of membrane insertion with protein function also emerged, with a higher extent of membrane insertion for pure water transporters compared to aquaporin family members with other substrate specificity. Nevertheless, the nucleic acid sequence of the second codon appears to play an important role in overproduction. Constructs containing guanine at the first position of this codon (being part of the mammalian Kozak sequence) are generally produced at a higher level, which is confirmed for hAQP8. In addition, mimicking the yeast consensus sequence (ATGTCT) apparently has a negative influence on the production level, as shown for hAQP1. Moreover, by mutational analysis we show that the yield of hAQP4 can be heavily improved by directing the protein folding pathway as well as stabilizing the aquaporin tetramer.

Biological

Immunoblotting

Humans

Models

genetics

chemistry

Phylogeny

metabolism

metabolism

Codon

Pichia

genetics

genetics

Aquaporins

classification

Författare

Fredrik Öberg

Göteborgs universitet

Mikael Ekvall

Göteborgs universitet

Anna Maria Nyblom

Chalmers, Kemi- och bioteknik, Molekylär mikroskopi

Anna Backmark

Chalmers, Kemi- och bioteknik

Richard Neutze

Göteborgs universitet

Kristina Hedfalk

Göteborgs universitet

Molecular Membrane Biology

0968-7688 (ISSN) 1464-5203 (eISSN)

Vol. 26 4 215-27

Ämneskategorier

Kemi

DOI

10.1080/09687680902862085

PubMed

19384754