Modification of nanocellulose with a xyloglucan-RGD conjugate enhances adhesion and proliferation of endothelial cells: implications for tissue engineering.
Artikel i vetenskaplig tidskrift, 2007

This paper describes a novel method for introducing the RGD cell adhesion peptide to enhance cell adhesion onto bacterial cellulose (BC). BC and cotton linters as reference were modified with xyloglucan (XG) and xyloglugan bearing a GRGDS pentapeptide. The adsorptions followed Langmuir adsorption behavior, where both XGs probably decorate the cellulose surfaces as a monolayer. The adsorption maximum of the XGs reached around 180 mg/g on BC and only about three times as much on cotton linters. The adsorption was verified with colorimetric methods. The specific surface area of BC measured with XG and XG-GRGDS was about 200 m (2)/g and was almost three times less for cotton linters, 60 m (2)/g. The difference in the amounts of XGs adsorbed might be explained by the swollen network of bacterial cellulose and a more exposed and accessible bulk as compared to cotton linters. The nanocellulose material was modified homogeneously throughout the material, as seen by the z-scan in confocal microscopy. Moreover, the modification in the water phase, in comparison with organic solvents, was clearly advantageous for preserving the morphology, as observed with SEM. The modification slightly increased the wettability, which might explain the decrease in or undetectable adsorption of adhesive protein shown by QCM-D. Initial cell studies showed that adhesion of human endothelial cells is enhanced when the BC hydrogel is modified with XG-GRGDS. QCM-D studies further revealed that the cell enhancement is due to the presence of the RGD epitope on XG and not to a nonspecific adsorption of fibronectin from cell culture medium. Optimization and proliferation studies of human endothelial cells onto bacterial cellulose modified with XG-GRGDS are currently being carried out at the Vascular Engineering Center, Sahlgrenska University Hospital, Gothenburg.

Endothelial Cells

Cell Adhesion

Cellulose

chemistry

chemistry

Xylans

metabolism

Humans

cytology

Cells

trends

metabolism

Oligopeptides

metabolism

physiology

Cell Proliferation

metabolism

methods

Nanoparticles

chemistry

metabolism

chemistry

chemistry

Glucans

Tissue Engineering

Cultured

Författare

Aase Katarina Bodin

Chalmers, Kemi- och bioteknik, Polymerteknologi

Lage Ahrenstedt

Kungliga Tekniska Högskolan (KTH)

Helen Fink

Göteborgs universitet

Harry Brumer

Kungliga Tekniska Högskolan (KTH)

Bo Risberg

Göteborgs universitet

Paul Gatenholm

Chalmers, Kemi- och bioteknik, Polymerteknologi

Biomacromolecules

1525-7797 (ISSN) 1526-4602 (eISSN)

Vol. 8 3697-3704

Ämneskategorier

MEDICIN OCH HÄLSOVETENSKAP

DOI

10.1021/bm070343q

PubMed

18031014