Single-file electrophoretic transport and counting of individual DNA molecules in surfactant nanotubes
Artikel i vetenskaplig tidskrift, 2005

We demonstrate a complete nanotube electrophoresis system (nanotube radii in the range of 50 to 150 nm) based on lipid membranes, comprising DNA injection, single-molecule transport, and single-molecule detection. Using gel-capped electrodes, electrophoretic single-file transport of fluorescently labeled dsDNA molecules is observed inside nanotubes. The strong confinement to a channel of molecular dimensions ensures a detection efficiency close to unity and identification of DNA size from its linear relation to the integrated peak intensity. In addition to constituting a nanotechnological device for identification and quantification of single macromolecules or biopolymers, this system provides a method to study their conformational dynamics, reaction kinetics, and transport in cell-like environments.

Författare

Michal Tokarz

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

Björn Åkerman

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

Jessica Olofsson

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

J. F. Joanny

Physico-Chimie Curie

P. Dommersnes

Physico-Chimie Curie

Owe Orwar

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

Proceedings of the National Academy of Sciences of the United States of America

0027-8424 (ISSN) 1091-6490 (eISSN)

Vol. 102 26 9127-9132

Ämneskategorier

Kemiteknik

DOI

10.1073/pnas.0500081102