Accumulation of FITC near stratum corneum-visualizing epidermal distribution of a strong sensitizer using two-photon microscopy
Artikel i vetenskaplig tidskrift, 2009

Background The allergenic potency of a hapten is related to its skin penetration properties, but little is known about the distribution of haptens in the skin following topical application. Objectives The aim of this study was to investigate the diffusion and epidermal distribution using two-photon microscopy (TPM) of two fluorescent compounds. Methods Sensitizing capacities of fluorescein isothiocyanate (FITC) and fluorescein were investigated using the local lymph node assay. Chemical reactivity of the compounds was analysed, and their distribution in human epidermis was visualized using TPM and confocal microscopy. Also the in vitro diffusion through epidermis of FITC and fluorescein has been examined. Results FITC was classified as an extreme sensitizer, whereas fluorescein was non-sensitizing. TPM and confocal microscopy showed an accumulation of FITC in stratum corneum (SC), whereas fluorescein was more evenly distributed in epidermis. The diffusion of fluorescein through epidermis was three times higher than that of FITC. Conclusions TPM, which has never been used in this context before, is a promising tool for visualizing the distribution of fluorescent compounds of varying reactivity in intact skin. The strong allergen FITC is mainly retained in or adjacent to SC, whereas most fluorescein diffused through the epidermis.

local lymph node assay

microscopy

peptide reactivity

fluorescein isothiocyanate

two-photon

laser scanning confocal

allergic contact-dermatitis

contact allergy

sensitization

microscopy

phenylisothiocyanate

skin penetration

classification

in-vitro

fluorescent hapten

skin

hypersensitivity

FITC

dinitrochlorobenzene

lymph-node assay

Författare

Kristin Samuelsson

Göteborgs universitet

Carl Simonsson

Göteborgs universitet

Charlotte A Jonsson

Göteborgs universitet

Gunnar Westman

Chalmers, Kemi- och bioteknik, Organisk kemi

Marica B Ericson

Göteborgs universitet

Ann-Therese Karlberg

Göteborgs universitet

Contact Dermatitis

0105-1873 (ISSN) 1600-0536 (eISSN)

Vol. 61 2 91-100

Ämneskategorier

Kemi

Organisk kemi

DOI

10.1111/j.1600-0536.2009.01591.x