Protein adsorption and desorption on lipid bilayers
Artikel i vetenskaplig tidskrift, 2010

The protein surface usually exhibits one or a few charged spots. If a lipid bilayer contains a significant amount of lipids with oppositely charged head groups, protein adsorption on a bilayer may be energetically favourable due to the protein-lipid electrostatic interaction. The specifics of this case are that the lipids are highly mobile and the protein adsorption is accompanied by the redistribution of lipids between the areas covered and not covered by protein. We present a kinetic model illustrating that this effect is especially interesting if the fraction of the surface covered by charged lipids is relatively low. In this situation, with increasing protein coverage, the protein desorption rate constant rapidly increases while the adsorption rate constant drops, so that there is critical fraction of the area covered by protein. Adsorption above this fraction is hindered both kinetically and thermodynamically.

charged

monte-carlo simulations

solid-surfaces

membranes

Lipids

Adsorption and desorption kinetics

Lipid

macromolecules

phospholipid-bilayers

redistribution

electrostatics

Proteins

excluded surface-area

kinetics

Electrostatic interactions

aggregation

qcm-d

Författare

Vladimir Zhdanov

Chalmers, Teknisk fysik, Kemisk fysik

Bengt Herbert Kasemo

Chalmers, Teknisk fysik, Kemisk fysik

Biophysical Chemistry

0301-4622 (ISSN)

Vol. 146 60-64

Ämneskategorier

Biokemi och molekylärbiologi

DOI

10.1016/j.bpc.2009.10.005