Intravital fluorescent microscopic evaluation of bacterial cellulose as scaffold for vascular grafts.
Artikel i vetenskaplig tidskrift, 2010

Although commonly used synthetic vascular grafts perform satisfactorily in large caliber blood vessels, they are prone to thrombosis in small diameter vessels. Therefore, small vessels might benefit from tissue engineered vascular grafts. This study evaluated bacterial cellulose (BC) as a potential biomaterial for biosynthetic blood vessels. We implanted the dorsal skinfold chambers in three groups of Syrian golden hamsters with BC (experimental group), polyglycolic acid, or expanded polytetrafluorethylene (control groups). Following implantation, we used intravital fluorescence microscopy, histology, and immunohistochemistry to analyze the biocompatibility, neovascularization, and incorporation of each material over a time period of 2 weeks. Biocompatibility was good in all groups, as indicated by the absence of leukocyte activation upon implantation. All groups displayed angiogenic response in the host tissue, but that response was highest in the polyglycolic acid group. Histology revealed vascularized granulation tissue surrounding all three biomaterials, with many proliferating cells and a lack of apoptotic cell death 2 weeks after implantation. In conclusion, BC offers good biocompatibility and material incorporation compared with commonly used materials in vascular surgery. Thus, BC represents a promising new biomaterial for tissue engineering of vascular grafts.

Blood Vessel Prosthesis

drug effects

pathology

Acetobacter

Inflammation

Neovascularization

Immunohistochemistry

Cricetinae

drug effects

chemistry

Fluorescence

Mesocricetus

Leukocyte Rolling

Female

Tissue Scaffolds

pharmacology

drug effects

Microscopy

methods

drug effects

Animals

Hemodynamics

Physiologic

Cellulose

Materials Testing

physiology

drug effects

chemistry

Cell Adhesion

Microvessels

Författare

Maricris Esguerra

Göteborgs universitet

Helen Fink

Göteborgs universitet

Matthias W Laschke

Universitatsklinikum des Saarlandes Medizinische Fakultat der Universitat des Saarlandes

Anders Jeppsson

Göteborgs universitet

Dick Delbro

Göteborgs universitet

Paul Gatenholm

Chalmers, Kemi- och bioteknik, Polymerteknologi

Michael D Menger

Universitatsklinikum des Saarlandes Medizinische Fakultat der Universitat des Saarlandes

Bo Risberg

Göteborgs universitet

Journal of biomedical materials research. Part A

1552-4965 (eISSN)

Vol. 93 140-9

Ämneskategorier

Kirurgi

MEDICIN OCH HÄLSOVETENSKAP

DOI

10.1002/jbm.a.32516

PubMed

19536832