Bacterial Cellulose as a Potential Scaffold for Tissue Engineering of Cartilage
Artikel i vetenskaplig tidskrift, 2005

Tissue constructs for cartilage with native mechanical properties have not been described to date. To address this need the bacterial cellulose (BC) secreted by Gluconacetobacter xylinus (= Acetobacter xylinum) was explored as a novel scaffold material due to its unusual material properties and degradability. Native and chemically modified BC materials were evaluated using bovine chondrocytes. The results indicate that unmodified BC supports chondrocyte proliferation at levels of approximately 50% of the collagen type II substrate while providing significant advantages in terms of mechanical properties. Compared to tissue culture plastic and calcium alginate, unmodified BC showed significantly higher levels of chondrocyte growth. Chemical sulfation and phosphorylation of the BC, performed to mimic the glucosaminoglycans of native cartilage, did not enhance chondrocyte growth while the porosity of the material did affect chondrocyte viability. The BC did not induce significant activation of proinflammatory cytokine production during in vitro macrophage screening. Hence, unmodified BC was further explored using human chondrocytes. TEM analysis and RNA expression of the collagen II from human chondrocytes indicated that unmodified BC supports proliferation of chondrocytes. In addition, ingrowth of chondrocytes into the scaffold was verified by TEM. The results suggest the potential for this biomaterial as a scaffold for tissue engineering of cartilage

Elasticity

metabolism

cytology

Cattle

Articular

Cell Differentiation

Tissue Engineering

Chondrocytes

growth & development

Cell Size

chemistry

Humans

physiology

cytology

physiology

Tensile Strength

Cell Adhesion

Compressive Strength

Feasibility Studies

methods

Materials Testing

Animals

Cellulose

Gluconacetobacter xylinus

Cell Proliferation

Cartilage

Biocompatible Materials

Cell Survival

chemistry

physiology

Författare

Anna Svensson

Chalmers University of Technology

Tufts University

Elin Nicklasson

Chalmers, Kemi- och bioteknik, Polymerteknologi

Tim Harrah

Tufts University

B Panilaitis

Tufts University

David Kaplan

Tufts University

Mats Brittberg

Göteborgs universitet

Paul Gatenholm

Chalmers, Kemi- och bioteknik, Polymerteknologi

Biomaterials

0142-9612 (ISSN)

Vol. 26 4 419-431

Ämneskategorier

Annan materialteknik

DOI

10.1016/j.biomaterials.2004.02.049