Nonlinear microscopy with fiber laser continuum excitation
Artikel i vetenskaplig tidskrift, 2010

A compact high-power fiber-based femtosecond laser system is presented for coherent anti-Stokes Raman scattering/second-harmonic generation (CARS/SHG) microscopy, and quantitatively compared with a conventional picosecond optical parametric oscillator (OPO)-based system. While the broad spectral width of the femtosecond pulses results in 2.5 times lower image contrast and limited spectral selectivity, lipid stores, myosin, and collagen filaments in living cells can clearly be identified at 60 times lower excitation powers compared to the picosecond system. Visually the images contain the same information. Together with simple operation, small footprint, and low cost, the capabilities of this high-power all-fiber-based laser system promise a more general use of nonlinear microscopy within the biosciences. (C) 2010 Society of Photo-Optical Instrumentation Engineers. [DOI: 10.1117/1.3374039]

nonlinear microscopy

cars microscopy

fiber-based laser

femtosecond laser

raman scattering microscopy

cells

photodamage

system

2nd-harmonic generation

light-source

Författare

Fredrik Svedberg

Chalmers, Kemi- och bioteknik, Molekylär mikroskopi

Christian Brackmann

Chalmers, Kemi- och bioteknik, Molekylär mikroskopi

Thomas Hellerer

TOPTICA Photonics AG

Annika Enejder

Chalmers, Kemi- och bioteknik, Molekylär mikroskopi

Journal of Biomedical Optics

1083-3668 (ISSN)

Vol. 15 2 Art. Nr. 026026-

Ämneskategorier

Kemi

DOI

10.1117/1.3374039

PubMed

20459271