Microporous bacterial cellulose as a potential scaffold for bone regeneration
Artikel i vetenskaplig tidskrift, 2010

Nanoporous cellulose biosynthesized by bacteria is an attractive biomaterial scaffold for tissue engineering due to its biocompatibility and good mechanical properties. However, for bone applications a microscopic pore structure is needed to facilitate osteoblast ingrowth and formation of a mineralized tissue. Therefore, in this study microporous bacterial cellulose (BC) scaffolds were prepared by incorporating 300-500 mu m paraffin wax microspheres into the fermentation process. The paraffin wax microspheres were subsequently removed, and scanning electron microscopy confirmed a microporous surface of the scaffolds while Fourier transform infrared spectroscopy verified the elimination of paraffin and tensile measurements showed a Young's modulus of approximately 1.6 MPa. Microporous BC and nanoporous (control) BC scaffolds were seeded with MC3T3-E1 osteoprogenitor cells, and examined by confocal microscopy and histology for cell distribution and mineral deposition. Cells clustered within the pores of microporous BC, and formed denser mineral deposits than cells grown on control BC surfaces. This work shows that microporous BC is a promising biomaterial for bone tissue engineering applications. (C) 2010 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.

biomimetic synthesis

differentiation

Bacterial cellulose

biomaterials

Mineralization

Porogen

Bone tissue engineering

proliferation

MC3T3-E1

cells

Författare

Magdalena Zaborowska

Chalmers, Kemi- och bioteknik, Polymerteknologi

Aase Katarina Bodin

Chalmers, Kemi- och bioteknik, Polymerteknologi

Henrik Bäckdahl

Chalmers, Kemi- och bioteknik, Polymerteknologi

J. Popp

Virginia Polytechnic Institute and State University

A. Goldstein

Virginia Polytechnic Institute and State University

Paul Gatenholm

Chalmers, Kemi- och bioteknik, Polymerteknologi

Acta Biomaterialia

1742-7061 (ISSN)

Vol. 6 7 2540-2547

Ämneskategorier

Odontologi

Kemi

DOI

10.1016/j.actbio.2010.01.004