Leukocyte oxygen radical production determines disease severity in the recurrent Guillain-Barré
Artikel i vetenskaplig tidskrift, 2010
Background: The recurrent Guillain-Barré syndrome (RGBS) is characterized by at least two GBS episodes with
intervening remission. In a previous study of monophasic GBS, we reported that the magnitude of oxygen radical
production ("respiratory burst”) in peripheral blood leukocytes was inversely correlated to disease severity. The
present study sought to establish a similar correlation in patients with RGBS.
Methods: Oxygen radical production in leukocytes was induced by formyl-Met-Leu-Phe (fMLF), Trp-Lys-Tyr-Met-Val-
Met-NH2 (WKYMVM), or phorbol myristate acetate (PMA) and assessed by quantifying superoxide anion formed by
the leukocyte NADPH oxidase.
Results: Disease severity, assessed using the MRC score, was negatively correlated to superoxide anion production
triggered by fMLF or WKYMVM (p = 0.001 and 0.002, respectively; n = 10). Superoxide anion production also was
significantly lower in RGBS patients with incomplete recovery after stimulation with fMLF (p = 0.004) or WKYMVM
(p = 0.003).
Conclusion: We conclude that a lower respiratory burst in leukocytes is strongly associated with a severe course of
oxygen radical production