Cell-Based Assay To Quantify the Antioxidant Effect of Food-Derived Carotenoids Enriched in Postprandial Human Chylomicrons
Artikel i vetenskaplig tidskrift, 2010

We developed a new method to evaluate the antioxidant effect of food products in a biological system. The antioxidant status of HepG2 cells was quantified after incubation with postprandial human chylomicrons after the intake of vegetable products. Three subjects consumed in a meal a vegetable soup containing 8.4 mg of beta-carotene and 9 mg of lycopene. After 5 h, the subjects consumed a second meal without carotenoids. Blood samples were collected at basal time and every hour for 9 h. Chylomicrons were isolated from serum samples and used for both carotenoid quantification and HepG2 stimulation. Carotenoid in chylomicrons followed an inter-individual and bimodal carotenoid response. We demonstrated the antioxidant effect of postprandial chylomicrons in HepG2 at the time of maximum carotenoid concentration of chylomicrons with respect to basal time. This cell-based assay seems to be a useful method to evaluate the antioxidant effect of fruit and vegetable products in a biological system.

lycopene

postprandial

chylomicron

beta-carotene

metabolism

salmonella-typhimurium

HepG2 cells

remnants

hepg2 cells

dietary

plasma

tomato lycopene

oxidative stress

bioavailability

vegetable and fruit food

Reactive oxygen species

beta-carotene

Författare

M. Sanchez-Campillo

Universidad de Murcia

F. Perez-Llamas

Universidad de Murcia

D. Gonzalez-Silvera

Universidad de Murcia

R. Martinez-Tomas

Universidad de Murcia

M. I. Burgos

Hospital Virgen de la Arrixaca

Anna Wellner

Chalmers, Kemi- och bioteknik, Livsvetenskaper, Livsmedelsvetenskap

F. Aviles

Hospital Virgen de la Arrixaca

S. Parra

Hospital Virgen de la Arrixaca

L Bialek

Unilever R&D Vlaardingen

Marie Alminger

Chalmers, Kemi- och bioteknik, Livsvetenskaper, Livsmedelsvetenskap

E. Larque

Universidad de Murcia

Journal of Agricultural and Food Chemistry

0021-8561 (ISSN) 1520-5118 (eISSN)

Vol. 58 20 10864-10868

Ämneskategorier

Livsmedelsvetenskap

Kemi

Styrkeområden

Livsvetenskaper och teknik

DOI

10.1021/jf102627g