Cell-Based Assay To Quantify the Antioxidant Effect of Food-Derived Carotenoids Enriched in Postprandial Human Chylomicrons
Journal article, 2010
We developed a new method to evaluate the antioxidant effect of food products in a biological system. The antioxidant status of HepG2 cells was quantified after incubation with postprandial human chylomicrons after the intake of vegetable products. Three subjects consumed in a meal a vegetable soup containing 8.4 mg of beta-carotene and 9 mg of lycopene. After 5 h, the subjects consumed a second meal without carotenoids. Blood samples were collected at basal time and every hour for 9 h. Chylomicrons were isolated from serum samples and used for both carotenoid quantification and HepG2 stimulation. Carotenoid in chylomicrons followed an inter-individual and bimodal carotenoid response. We demonstrated the antioxidant effect of postprandial chylomicrons in HepG2 at the time of maximum carotenoid concentration of chylomicrons with respect to basal time. This cell-based assay seems to be a useful method to evaluate the antioxidant effect of fruit and vegetable products in a biological system.
HepG2 cells
dietary
beta-carotene
salmonella-typhimurium
Reactive oxygen species
tomato lycopene
postprandial
lycopene
remnants
hepg2 cells
chylomicron
beta-carotene
oxidative stress
vegetable and fruit food
metabolism
bioavailability
plasma
Author
M. Sanchez-Campillo
University of Murcia
F. Perez-Llamas
University of Murcia
D. Gonzalez-Silvera
University of Murcia
R. Martinez-Tomas
University of Murcia
M. I. Burgos
Hospital Virgen de la Arrixaca
Anna Wellner
Chalmers, Chemical and Biological Engineering, Life Sciences
F. Aviles
Hospital Virgen de la Arrixaca
S. Parra
Hospital Virgen de la Arrixaca
L Bialek
Unilever
Marie Alminger
Chalmers, Chemical and Biological Engineering, Life Sciences
E. Larque
University of Murcia
Journal of Agricultural and Food Chemistry
0021-8561 (ISSN) 1520-5118 (eISSN)
Vol. 58 20 10864-10868Subject Categories
Food Science
Chemical Sciences
Areas of Advance
Life Science Engineering (2010-2018)
DOI
10.1021/jf102627g