Tissue-engineered conduit using urine-derived stem cells seeded bacterial cellulose polymer in urinary reconstruction and diversion
Artikel i vetenskaplig tidskrift, 2010

The objective of this study was to generate bacterial cellulose (BC) scaffolds seeded with human urine-derived stem cells (USC) to form a tissue-engineered conduit for use in urinary diversion. Microporous BC scaffolds were synthesized and USC were induced to differentiate into urothelial and smooth muscle cells (SMC). Induced USC (10 6 cells/cm 2 ) were seeded onto BC under static and 3D dynamic (10 or 40 RPM) conditions and cultured for 2 weeks. The urothelial cells and SMC derived from USC formed multilayers on the BC scaffold surface, and some cells infiltrated into the scaffold. The urothelium derived from USC differentiation expressed urothelial markers (uroplakin Ia and AE1/AE3) and the SMC expressed SMC markers (α-smooth muscle actin and desmin). In addition, USC/BC scaffold constructs were implanted into athymic mice, and the cells were tracked using immunohistochemical staining for human nuclear antigen. In vivo, the cells appeared to differentiate and express urothelial and SMC markers. In conclusion, porous BC scaffolds allow 3 dimensional growth of USC, leading to formation of a multilayered urothelium and cell-matrix infiltration. Thus, cell-seeded BC scaffolds hold promise for use in tissue-engineered urinary conduits for urinary reconstruction. © 2010 Elsevier Ltd.

Urinary tract

Tissue-engineered urinary conduit


Mesenchcymal stem cells

Cell-matrix infiltration


Aase Katarina Bodin

Chalmers, Kemi- och bioteknik, Polymerteknologi

S. Bharadwaj

Wake Forest University

S. Wu

Wake Forest University

Paul Gatenholm

Chalmers, Kemi- och bioteknik, Polymerteknologi

A. Atala

Wake Forest University

Y. Zhang

Wake Forest University


0142-9612 (ISSN) 18785905 (eISSN)

Vol. 31 34 8889-8901


Industriell bioteknik



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