Co-Ion Dependence of DNA Nuclease Activity Suggests Hydrophobic Cavitation as a Potential Source of Activation Energy
Artikel i vetenskaplig tidskrift, 2001

The source of the activation energy that allows cutting of DNA by restriction enzymes is unclear. A systematic study of the cutting efficiency of the type-II restriction endonuclease EcoRI, with varying background electrolyte ion pair and buffer reported here, shows only a modest dependence of efficiency on cation type. Surprisingly, efficiency does depend strongly on the presumed indifferent anion of the background salt. What emerges is that competition between the background salt anion and the buffer anion for the enzyme and DNA surfaces is crucial. The results are unexpected and counterintuitive from the point of view of conventional electrolyte theory. However, taken together with recent developments in surface chemistry, the results do fall into place and could also suggest a novel mechanism for enzyme activity as an alternative to metal-activated hydrolysis: microscopic cavitation in a hydrophobic pocket might be the source of activation energy.

Författare

Hye-Kyung Kim

Institutionen för fysikalisk kemi

Eimer Tuite

Bengt Nordén

Institutionen för fysikalisk kemi

B.W. Ninham

European Physical Journal E

1292-8941 (ISSN) 1292-895X (eISSN)

Vol. 4 4 411-417

Styrkeområden

Nanovetenskap och nanoteknik

Energi

Livsvetenskaper och teknik

Materialvetenskap

Ämneskategorier

Fysikalisk kemi

Fundament

Grundläggande vetenskaper

DOI

10.1007/s101890170096

Mer information

Skapat

2017-10-07