Dissociation of non-complementary second DNA strand from RecA filament without ATP hydrolysis: mechanism of the search for homologous DNA
Artikel i vetenskaplig tidskrift, 1997

RecA protein catalyzes the DNA annealing and mimics the DNA strand exchange reaction in vitro in the presence of ATP or its non-hydrolyzable analog, adenosine 5'-O-3-thiotriphosphate (ATP gamma S). For these activities RecA coordinates two DNA molecules [Takahashi, M. and Norden, B. (1994) Adv. Biophys, 30, 1-35]. In order to get a better understanding of how RecA performs the search for sequence complementarity or homology between two DNA molecules, the association and dissociation kinetics oa second DNA molecule to and from RecA in the presence of ATP gamma S have been investigated, The kinetics were monitored by fluorescence measurements of partly etheno-modified poly(dA) assisted by linear dichroism measurements of the flow-oriented complex. The association of the second DNA is fast;, regardless of whether the sequence is complementary or not. Bg contrast, the dissociation kinetics is strongly dependent on sequence complementarity, If the second DNA is complementary to the first, dissociation is extremely slow, whilst that of non-complementary second DNA is fast, In no case does the first DNA leave the RecA fiber, Our findings indicate that the dissociation step is important in the search for homology by RecA.

homologous recombination

search of homologous DNA

strand exchange

RecA protein

DNA binding


C. Ellouze

Bengt Nordén

Institutionen för fysikalisk kemi

M. Takahashi

Journal of Biochemistry

0021-924X (ISSN)

Vol. 121 1070-1075


Nanovetenskap och nanoteknik


Livsvetenskaper och teknik



Fysikalisk kemi


Grundläggande vetenskaper