Accumulation and Separation of Membrane-Bound Proteins Using Hydrodynamic Forces
Artikel i vetenskaplig tidskrift, 2011

The separation of molecules residing in the cell membrane remains a largely unsolved problem in the fields of bioscience and biotechnology. We demonstrate how hydrodynamic forces can be used to both accumulate and separate membrane-bound proteins in their native state. A supported lipid bilayer (SLB) was formed inside a microfluidic channel with the two proteins streptavidin (SA) and cholera toxin (CT) coupled to receptors in the lipid bilayer. The anchored proteins were first driven toward the edge of the lipid bilayer by hydrodynamic forces from a flowing liquid above the SLB, resulting in the accumulation of protein molecules at the edge of the bilayer. After the concentration process, the bulk flow of liquid in the channel was reversed and the accumulated proteins were driven away from the edge of the bilayer. Each type of protein was found to move at a characteristic drift velocity, determined by the frictional coupling between the protein and the lipid bilayer, as well as the size and shape of the protein molecule. Despite having a similar molecular weight, SA and CT could be separated into monomolecular populations using this approach. The method also revealed heterogeneity among the CT-molecules, resulting in three subpopulations with different drift velocities. This was tentatively attributed to multivalent interactions between the protein and the monosialoganglioside G(M1) receptors in the lipid bilayer.

hydrophilic surfaces

complexes

supported lipid-bilayers

crystals

phospholipid-membranes

electrical manipulation

Författare

Peter Jönsson

Chalmers, Teknisk fysik, Biologisk fysik

Anders Gunnarsson

Chalmers, Teknisk fysik, Biologisk fysik

Fredrik Höök

Chalmers, Teknisk fysik, Biologisk fysik

Analytical Chemistry

0003-2700 (ISSN) 1520-6882 (eISSN)

Vol. 83 2 604-611

Ämneskategorier

Kemi

DOI

10.1021/ac102979b