Integrated Analysis of the Global Transcriptional Response to alpha-Amylase Over-Production by Aspergillus oryzae
Artikel i vetenskaplig tidskrift, 2011

The filamentous fungus Aspergillus oryzae is an important microbial cell factory for industrial production of many enzymes, such as alpha-amylase. In order to optimize the industrial enzyme production process, there is a need to understand fundamental processes underlying enzyme production, here under how enzyme production links to metabolism through global regulation. Through a genome-scale metabolic network for integrated analysis of transcriptome data and flux calculation, we identified key players (genes, enzymes, proteins, and metabolites) involved in the processes of enzyme synthesis and secretion, nucleotide metabolism, and amino acid metabolism that can be the potential targets for improving industrial enzyme production. Biotechnol. Bioeng. 2011;108: 1130-1139.

Aspergillus oryzae

saccharomyces-cerevisiae

systems biology

enzyme production

general control

alpha-amylase

amino-acid biosynthesis

microarray

probe level

data integration

Författare

Wanwipa Vongsangnak

Chalmers, Kemi- och bioteknik, Livsvetenskaper, Systembiologi

K. Hansen

Novozymes AS

Jens B Nielsen

Chalmers, Kemi- och bioteknik, Livsvetenskaper, Systembiologi

Biotechnology and Bioengineering

0006-3592 (ISSN) 1097-0290 (eISSN)

Vol. 108 1130-1139

Drivkrafter

Hållbar utveckling

Ämneskategorier

Industriell bioteknik

Styrkeområden

Energi

Livsvetenskaper och teknik

DOI

10.1002/bit.23033